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Genetics
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果蝇细胞中的核内高C
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Genetics
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JoVE Journal
Genetics
In-Nucleus Hi-C in Drosophila Cells
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Click here for the English version.
果蝇细胞中的核内高C
DOI:
10.3791/62106-v
•
11:58 min
•
September 15, 2021
•
Ayerim Esquivel-López
,
Rodrigo Arzate-Mejía
,
Rosario Pérez-Molina
,
Mayra Furlan-Magaril
1
Departamento de Genética Molecular, Instituto de Fisiología Celular
,
Universidad Nacional Autónoma de México
Chapters
00:05
Introduction
01:13
Schneiders Line 2 Plus (S2R+) Drosophila Cell Fixation
02:02
Lysis
03:18
Enzymatic Digestion, DNA End Biotinylation, and Ligation
04:37
Crosslink Reversal and DNA Purification
05:33
Hi-C Template Quality Evaluation
06:26
Biotin Removal/End Repair and Size Selection
07:49
Biotin Pulldown/A-Tailing/Adapter Ligation
09:33
Results: Representative In-Nucleus Hi-C Analysis
11:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
基因组在核空间中组织成不同的结构,可以通过染色体构象捕获技术揭示出来。核内Hi-C方法提供了在果蝇细胞系中全基因组的染色质相互作用集合,生成接触图,可以在限制片段水平下以兆基分辨率进行探索。
Tags
Hi-C
Chromatin Conformation
Genome Organization
Chromosomal Interactions
Drosophila Cells
Nuclear Clumps
Formaldehyde Crosslinking
Glycine Quenching
Cell Lysis
Restriction Enzyme Digestion
SDS Permeabilization
Triton Treatment
Quality Control
Sequencing
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