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果蝇细胞中的核内高C
JoVE Journal
Genetics
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JoVE Journal Genetics
In-Nucleus Hi-C in Drosophila Cells
DOI:

11:58 min

September 15, 2021

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Chapters

  • 00:05Introduction
  • 01:13Schneiders Line 2 Plus (S2R+) Drosophila Cell Fixation
  • 02:02Lysis
  • 03:18Enzymatic Digestion, DNA End Biotinylation, and Ligation
  • 04:37Crosslink Reversal and DNA Purification
  • 05:33Hi-C Template Quality Evaluation
  • 06:26Biotin Removal/End Repair and Size Selection
  • 07:49Biotin Pulldown/A-Tailing/Adapter Ligation
  • 09:33Results: Representative In-Nucleus Hi-C Analysis
  • 11:17Conclusion

Summary

Automatic Translation

基因组在核空间中组织成不同的结构,可以通过染色体构象捕获技术揭示出来。核内Hi-C方法提供了在果蝇细胞系中全基因组的染色质相互作用集合,生成接触图,可以在限制片段水平下以兆基分辨率进行探索。

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