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利用 体内 出生后电穿孔研究小脑颗粒神经元形态和突触发育
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
DOI:

04:20 min

June 09, 2021

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Chapters

  • 00:05Introduction
  • 00:39Preparing the Injection Syringe for Electroporation
  • 01:00Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
  • 02:34Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
  • 03:45Conclusion

Summary

Automatic Translation

在这里,我们描述了一种方法,当这些细胞细化其突触结构并形成突触以整合到整个脑回路中时,在出生后大脑发育的时间过程中可视化小鼠小脑中颗粒神经元的突触发生。

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