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Journal
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Immunology and Infection
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マウスから骨髄由来樹状細胞を単離および培養するための経済的かつ効率的なプロトコル
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
Please note that all translations are automatically generated.
Click here for the English version.
マウスから骨髄由来樹状細胞を単離および培養するための経済的かつ効率的なプロトコル
DOI:
10.3791/63125-v
•
04:29 min
•
July 01, 2022
•
Huiqin Tang*
1
,
Hui Xie*
1
,
Zhun Wang
,
Shuanghe Peng
,
Wenli Ni
,
Linpei Guo
1
Tianjin Institute of Urology
,
The Second Hospital of Tianjin Medical University
,
2
Department of Pathology
,
The Second Hospital of Tianjin Medical University
,
3
Department of Urology
,
The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University
Chapters
00:17
Introduction
00:37
Isolation of Bone Marrow and Preparation of BM Cells
02:05
Induction Culture of BMDC
03:11
Results
03:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、10 ng/mL GM-CSF/IL-4で7日間培養した後、マウスから高純度骨髄由来樹状細胞を単離・生成する経済的かつ効率的な方法を紹介します。
Tags
Keywords: Bone Marrow-derived Dendritic Cells
Isolation
Culture
Mouse
BMDC
CD11c
CD80
MSC2
GM-CSF
IL-4
Cost-effective
Efficient
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