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Økonomisk og effektiv protokol til isolering og dyrkning af knoglemarvsafledte dendritiske celler fra mus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Økonomisk og effektiv protokol til isolering og dyrkning af knoglemarvsafledte dendritiske celler fra mus

DOI:
10.3791/63125-v

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04:29 min

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July 01, 2022

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, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Chapters

  • 00:17Introduction
  • 00:37Isolation of Bone Marrow and Preparation of BM Cells
  • 02:05Induction Culture of BMDC
  • 03:11Results
  • 03:59Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en økonomisk og effektiv metode til at isolere og generere knoglemarvsafledte dendritiske celler med høj renhed fra mus efter 7 dages dyrkning med 10 ng/ml GM-CSF/IL-4.

Tags

Keywords: Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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