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Journal / Biology / De novo Identificatie van actief vertaalde open…
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JoVE Journal Biology
De novo Identificatie van actief vertaalde open leesframes met ribosoomprofileringsgegevens
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De novo Identificatie van actief vertaalde open leesframes met ribosoomprofileringsgegevens

Article DOI: 10.3791/63366-v • 08:23 min • February 18th, 2022
February 18th, 2022 •
*1, *2,3, 2,3, 1
1School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, 2MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, 3Joint Graduate Program of Peking-Tsinghua-National Institute of Biological Science
* These authors contributed equally

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Het vertalen van ribosomen decodeert drie nucleotiden per codon in peptiden. Hun beweging langs mRNA, vastgelegd door ribosoomprofilering, produceert de voetafdrukken die karakteristieke triplet periodiciteit vertonen. Dit protocol beschrijft hoe RiboCode te gebruiken om deze prominente functie te ontcijferen uit ribosoomprofileringsgegevens om actief vertaalde open leesframes op het niveau van het hele transcriptoom te identificeren.

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Biologie Ribosoomprofilering open leesframe mRNA-translatie micropeptide uORF dORF
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