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Biology
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从头 使用核糖体分析数据识别主动翻译的开放阅读框
 
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从头 使用核糖体分析数据识别主动翻译的开放阅读框

Article DOI: 10.3791/63366-v 08:23 min February 18th, 2022
February 18th, 2022

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Summary

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Click here for the English version.

翻译核糖体将每个密码子的三个核苷酸解码为肽。它们沿着mRNA的运动,通过核糖体分析捕获,产生表现出特征性三重态周期性的足迹。该协议描述了如何使用RiboCode从核糖体分析数据中破译这一突出特征,以识别全转录组水平上主动翻译的开放阅读框。

Tags

生物学,第180期,核糖体分析,开放阅读框架,mRNA翻译,微肽,uORF,dORF
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