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Journal
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Biology
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造血干细胞和祖细胞的CRISPR/Cas9基因编辑用于基因治疗应用
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JoVE Journal
Biology
CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
Please note that all translations are automatically generated.
Click here for the English version.
造血干细胞和祖细胞的CRISPR/Cas9基因编辑用于基因治疗应用
DOI:
10.3791/64064-v
•
08:32 min
•
August 09, 2022
•
Vigneshwaran Venkatesan*
1,2
,
Abisha Crystal Christopher*
1,3
,
Karthik V. Karuppusamy
2
,
Prathibha Babu
2
,
Manoj Kumar K. Alagiri
2
,
Saravanabhavan Thangavel
1
Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru
,
Christian Medical College campus
,
2
Manipal Academy of Higher Education
,
3
Thiruvalluvar University
Chapters
00:04
Introduction
00:53
Gene Editing of HSPCs
02:55
Transplantation of Gene-Edited HSPCs
04:39
Assessment of Short-Term Engraftment Potential
05:17
Assessment of Long-Term Engraftment Potential
07:01
Results: Evaluating the Efficiency of CRISPR/Cas9 Gene Editing of HSPCs
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了一种优化的造血干细胞和祖细胞(HSPC)培养程序,用于
体内基因
编辑细胞的稳健植入。
Tags
CRISPR/Cas9
Gene Editing
Hematopoietic Stem And Progenitor Cells
Gene Therapy
Ex Vivo Culture
Stemness Preservation
Monogenic Diseases
HIV
HSPC Gene Therapy Protocol
Nucleofection
NSG Mice
NBSGW Mice
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