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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
JoVE Journal
Genetics
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JoVE Journal Genetics
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
DOI:

08:27 min

May 21, 2018

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Chapters

  • 00:04Title
  • 01:01Mouse Acute Septic Lung Injury
  • 02:05Exosome Isolation and Purification
  • 03:41Exosome Recipient Uptake and Interaction Validation
  • 05:30Results: BALF-isolated Exosome Characterization
  • 06:53Conclusion

Summary

Automatic Translation

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

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