Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Coronavirus Free Access Resource Center

The science community is working hard to address the potential health crisis brought about by the outbreak of a novel coronavirus (COVID-19). To support these critical efforts, JoVE has made its coronavirus focused content and related resources freely available.

Corona Specific Protocols

General Virus Protocols

Lab Preparation

Additional Related Resources

Current Status and Research

It is unknown whether a pregnant woman infected by the “SARS-CoV-2” virus can pass it to her fetus, called “vertical transmission.” However, infants born to mothers affected by COVID-19 have displayed adverse health outcomes such as respiratory distress. The scientists are still not sure whether these adverse outcomes are related or not to the COVID-19 infection in their mothers.

Metagenomic RNA sequencing was used to first identify the coronavirus in a sample from a patient who had worked at the Wuhan food market. A draft genome sequence was made publicly available in the GenBank sequence repository on January 10th (last updated on January 17th).

A rapid-response team from the Chinese Center for Disease Control and Prevention isolated and cultivated the coronavirus from bronchoalveolar lavage fluid of three patients and identified it as the probable source of the outbreak

Microscopic techniques such as Correlative Light Electron Microscopy (CLEM), TEM or Negative Stain Electron Microscopy can be used to identify the morphology of coronavirus. Additionally, techniques such as Live Imaging using a Luciferase-fluorescent Reporter can be used to quantify the viral particles

Further genome sequencing revealed that the virus shared over 85% sequence identity with a known SARS-like coronavirus found in bats. The sequences were deposited in an open access database - Global Initiative on Sharing All Influenza Data (GISAID)

A group of scientists based in Wuhan and Beijing demonstrated that the virus uses angiotensin-converting enzyme II (ACE2) to gain entry to host cells (as did SARS-CoV)

Several international groups have been working from the open access sequence data to design primers for polymerase chain reaction (PCR) for diagnostics tests of SARS-CoV-2 (formerly 2019-nCoV)

Christian Drosten’s Lab at the Institute of Virology, Charité University Hospital, Berlin (with collaborators from Europe and Hong Kong) published a real-time PCR (RT-PCR) diagnostic test and workflow which detects SARS-CoV-2 and distinguishes it from SARS-CoV

A research group at Hong Kong University developed two one-step quantitative RT-PCR tests targeting both the open reading frame 1b (ORF1b) and the N regions of the viral genome. The N gene assay is recommended as a screening test and the ORF1b test is recommended as a confirmation test. (Reference: academic.oup.com/5719336)

Research methods

Current Diagnosis Methods

Treatment and prevention

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter