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Biology
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流动小鼠减数分裂细胞的流式细胞仪净化
JoVE Journal
Biology
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JoVE Journal
Biology
Flow Cytometry Purification of Mouse Meiotic Cells
Please note that all translations are automatically generated.
Click here for the English version.
流动小鼠减数分裂细胞的流式细胞仪净化
DOI:
10.3791/2602-v
•
10:43 min
•
April 15, 2011
•
Irina V. Getun
,
Bivian Torres
,
Philippe R.J. Bois
1
Genome Plasticity Laboratory, Department of Cancer Biology
,
The Scripps Research Institute
,
2
Flow Cytometry Core
,
The Scripps Research Institute
Chapters
00:05
Title
02:03
Testis Dissociation and Hoechst 33342 Staining of Testicular Cells
06:03
FACS Setup and Purification
08:30
Results of Flow Cytometry Purification
09:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种有效的方法,从小鼠睾丸中获得高度纯化的可行减数分裂分数描述,一个精细胞分解协议相结合的荧光激活细胞分选(FACS)。这种方法需要利用DNA含量和核密度的离散减数分裂分数的差异。
Tags
Flow Cytometry
Purification
Mouse Meiotic Cells
Testis
Differentiation Programs
Elutriation
Staput
BSA Gradient
Percoll Gradient
Cell Size
Cell Density
Meiotic Fractions
Molecular Analyses
FACS
Hoechst 33342 Staining
Germ Cells
Round Spermatids
Viable Cells
Meiosis Progression
Gene Expression
Nucleosome Occupancy
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