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流动小鼠减数分裂细胞的流式细胞仪净化
JoVE Journal
Biology
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JoVE Journal Biology
Flow Cytometry Purification of Mouse Meiotic Cells
DOI:

10:43 min

April 15, 2011

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Chapters

  • 00:05Title
  • 02:03Testis Dissociation and Hoechst 33342 Staining of Testicular Cells
  • 06:03FACS Setup and Purification
  • 08:30Results of Flow Cytometry Purification
  • 09:58Conclusion

Summary

Automatic Translation

一种有效的方法,从小鼠睾丸中获得高度纯化的可行减数分裂分数描述,一个精细胞分解协议相结合的荧光激活细胞分选(FACS)。这种方法需要利用DNA含量和核密度的离散减数分裂分数的差异。

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