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ゲートウェイ互換二分子蛍光相補(BiFC)システムを使用してプラントにおけるタンパク質相互作用の検出
Chapters
Summary
Please note that all translations are automatically generated.
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我々は、植物におけるタンパク質間相互作用をテストする手法を開発した。黄色蛍光タンパク質(YFP)がつの非オーバーラップフラグメントに分割されます。各断片は融合タンパク質の発現を可能にする、ゲートウェイのシステムを経由して目的の遺伝子にインフレームでクローニングされる。検死のタンパク質が相互作用するときにYFPシグナルの再構成にのみ発生します。
Tags
植物の生物学、問題55、タンパク質相互作用、ゲートウェイ、二分子蛍光相補、共焦点顕微鏡、アグロバクテリウム、ニコチbenthamiana、シロイヌナズナRelated Videos
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