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使用网关兼容双分子荧光互补(附设)系统在植物蛋白相互作用检测
 
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使用网关兼容双分子荧光互补(附设)系统在植物蛋白相互作用检测

Article DOI: 10.3791/3473
September 16th, 2011

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Summary September 16th, 2011

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我们已经开发出一种技术,以测试植物蛋白质相互作用。一个黄色荧光蛋白(YFP)被分成两个非重叠的片段。每个帧通过网关系统的兴趣基因片段克隆,使融合蛋白的表达。重组YFP的信号只发生在研讯的蛋白质相互作用。

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