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人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型
JoVE Journal
Medicine
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JoVE Journal Medicine
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
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人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型

DOI:
10.3791/4218-v

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12:04 min

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August 14, 2012

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, , , ,
1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey,University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences,Institute for Advanced Study, Princeton, New Jersey

Chapters

  • 00:05Title
  • 02:05Preparation of 1% Agarose-coated 24-well Plates
  • 03:08Preparation of Single Cell Suspensions
  • 04:17Spheroid Culture
  • 05:31Drug Treatment and Monitoring of 3D Spheroid Cultures
  • 07:22HistoGel-embedding of the NET 3D Spheroids
  • 09:06Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出一个简单的琼脂糖凝胶覆盖的平台，成长的3D使用神经内分泌肿瘤细胞株的多细胞球体。这种方法提供了一个非常方便的方法来研究神经内分泌肿瘤细胞的治疗药物的效果。它也可以帮助我们建立人类癌症治疗神经内分泌肿瘤球体。

Tags

Neuroendocrine TumorHuman Cell LinesThree-dimensional ModelStudyTherapyIncidenceMalignanciesSystemic TherapyBiologyTherapeutic TargetsNonresectableMetastatic Neuroendocrine Tumors3D Cell CultureDrug ScreeningMicroenvironmentMulticellular SpheroidsIn Vitro ModelAgarose-coated PlatePhysiological ConditionsDrug Treatments

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