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Humane Neuroendokrine tumorcellelinier som en tredimensional model for studiet af den menneskelige Neuroendokrine Tumor Therapy
JoVE Journal
Medicine
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JoVE Journal Medicine
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Humane Neuroendokrine tumorcellelinier som en tredimensional model for studiet af den menneskelige Neuroendokrine Tumor Therapy

DOI:
10.3791/4218-v

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12:04 min

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August 14, 2012

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, , , ,
1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey,University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences,Institute for Advanced Study, Princeton, New Jersey

Chapters

  • 00:05Title
  • 02:05Preparation of 1% Agarose-coated 24-well Plates
  • 03:08Preparation of Single Cell Suspensions
  • 04:17Spheroid Culture
  • 05:31Drug Treatment and Monitoring of 3D Spheroid Cultures
  • 07:22HistoGel-embedding of the NET 3D Spheroids
  • 09:06Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en simpel agarose overlay platform at vokse 3D flercellede kugler med neuroendokrine cancercellelinjer. Denne fremgangsmåde tilvejebringer en meget bekvem måde at undersøge virkningen af ​​terapeutiske lægemidler på neuroendokrine tumorceller. Det kunne også hjælpe os med at etablere menneskelige neuroendokrine tumorer sfæroiderne for kræftbehandling.

Tags

Neuroendocrine TumorHuman Cell LinesThree-dimensional ModelStudyTherapyIncidenceMalignanciesSystemic TherapyBiologyTherapeutic TargetsNonresectableMetastatic Neuroendocrine Tumors3D Cell CultureDrug ScreeningMicroenvironmentMulticellular SpheroidsIn Vitro ModelAgarose-coated PlatePhysiological ConditionsDrug Treatments

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