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Journal / Immunology and Infection / Høy gjennomstrømming Screening for Bredspektret…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Høy gjennomstrømming Screening for Bredspektret Chemical hemmere av RNA-virus
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Høy gjennomstrømming Screening for Bredspektret Chemical hemmere av RNA-virus

Article DOI: 10.3791/51222-v • 11:34 min • May 5th, 2014
May 5th, 2014 •
1, 2, 2, 1, 3, 1, 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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In vitro assays for å måle virusreplikasjon er blitt betydelig forbedret ved utvikling av rekombinante RNA-virus som uttrykker luciferase, eller andre enzymer som er i stand til bioluminesens. Her vi detalj en high-throughput screening rørledning som kombinerer slike rekombinante stammer av meslinger og chikungunya virus å isolere bredspektret antivirale midler fra kjemiske biblioteker.

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Immunologi virusinfeksjoner high-throughput screening-analyser bredspektret antivirale midler chikungunya virus meslinger virus luciferase reporter kjemiske biblioteker
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