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Journal
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Medicine
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乳腺癌细胞侵袭定量使用三维(3D)模型
JoVE Journal
Medicine
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JoVE Journal
Medicine
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
Please note that all translations are automatically generated.
Click here for the English version.
乳腺癌细胞侵袭定量使用三维(3D)模型
DOI:
10.3791/51341-v
•
08:08 min
•
June 11, 2014
•
Donna Cvetković*
1
,
Cameron Glenn-Franklin Goertzen*
1
,
Moshmi Bhattacharya
2,3
1
Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry
,
University of Western Ontario
,
2
Department of Oncology, Schulich School of Medicine and Dentistry
,
University of Western Ontario
,
3
Lawson Health Research Institute
Chapters
00:05
Title
01:06
3D Culture of Breast Cancer Cells in Basement Membrane Matrix
03:58
Examination of Morphogenic Features of 3D Cultures with Immunofluorescence
06:17
Results: Representative 3D MDA-MB-231 Cell Basement Membrane Matrix Invasion Images
07:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供的详细方法为使用三维(3D)测定法来量化乳腺癌细胞的侵袭。具体来说,我们将讨论建立这样的检测要求的程序,量化和数据分析,以及方法来探讨及细胞膜的完整性,当细胞侵入发生的损失。
Tags
Breast Cancer
3D Cell Culture
Extracellular Matrix
Invasion
Morphology
Immunostaining
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