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结合DNA-RNA荧光<em>原位</em>杂交(FISH)研究X染色体失活的分化雌性小鼠胚胎干细胞
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Biology
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JoVE Journal
Biology
Combined DNA-RNA Fluorescent
In situ
Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
结合DNA-RNA荧光<em>原位</em>杂交(FISH)研究X染色体失活的分化雌性小鼠胚胎干细胞
DOI:
10.3791/51628-v
•
15:54 min
•
June 14, 2014
•
Tahsin Stefan Barakat
,
Joost Gribnau
1
Department of Reproduction and Development
,
Erasmus MC – University Medical Center
Chapters
00:05
Title
02:57
Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
04:55
Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
05:33
Labeling of DNA Probes for FISH Experiments
08:02
Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
11:04
Post-hybridization Washes and Antibody Mediated Detection of Probe
13:21
Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
14:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
荧光
原位
杂交(FISH)可在细胞内其天然环境中的核酸的检测。我们在这里描述了一种协议,用于组合,同时检测RNA和DNA的鱼的装置,它可以被用来研究X染色体失活在小鼠胚胎干细胞。
Tags
DNA FISH
RNA FISH
Combined DNA-RNA FISH
X Chromosome Inactivation
Xist RNA
Female Mouse Embryonic Stem Cells
Gene Expression Regulation
Cytogenetics
Cancer Diagnostics
Genome Aberrations
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