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Visualisering af Thalamocortical Axon forgrening og Synapse dannelse i Organotypic Cocultures
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Visualisering af Thalamocortical Axon forgrening og Synapse dannelse i Organotypic Cocultures

DOI:
10.3791/56553-v

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06:16 min

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March 28, 2018

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1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Chapters

  • 00:04Title
  • 00:58Preparation of Cortical Slices
  • 02:24Procedure of Electroporation
  • 04:37Results: Thalamocortical Axons Coculture and Time-lapse Imaging
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en metode til samtidige billeddannelse af thalamocortical axon forgrening og synapse dannelse i organotypic cocultures af thalamus og hjernebarken. Enkelte thalamocortical axoner og deres præsynaptiske terminaler er visualiseret ved en enkelt celle elektroporation teknik med DsRed og normal god landbrugspraksis-mærket synaptophysin.

Tags

ThalamocorticalAxon BranchingSynapse FormationOrganotypic CoculturesNeural Circuit FormationAxon GrowthSynapse FormationCerebral CortexPostnatal Day-two RatPrimary Visual CortexSomatosensory CortexCulture InsertThalamic BlockE15 EmbryoGlass CapillariesElectrode PipettePlasmid Injection

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