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DNA甲基化:亚硫酸氢修改和分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
DNA Methylation: Bisulphite Modification and Analysis
DOI:

12:34 min

October 21, 2011

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Chapters

  • 00:05Title
  • 01:52DNA Preparation and Denaturation
  • 02:57Bisulphite Deamination
  • 06:38PCR Amplification
  • 09:08Results of DNA Methylation Analysis
  • 12:15Conclusion

Summary

Automatic Translation

DNA甲基化分析的黄金标准是亚硫酸氢转换的DNA基因组测序。 5 - 甲基胞嘧啶(5 - MEC)亚硫酸氢在酸性条件下脱氨相比,这种方法需要利用胞嘧啶的敏感性增加。后PCR扩增目标基因组DNA甲基化的胞嘧啶甲基化的胞嘧啶可以区分。

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