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Journal / Biology / DNA甲基化：亚硫酸氢修改和分析
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Biology

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DNA甲基化：亚硫酸氢修改和分析
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DNA甲基化：亚硫酸氢修改和分析

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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Summary
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DNA甲基化分析的黄金标准是亚硫酸氢转换的DNA基因组测序。 5 - 甲基胞嘧啶（5 - MEC）亚硫酸氢在酸性条件下脱氨相比，这种方法需要利用胞嘧啶的敏感性增加。后PCR扩增目标基因组DNA甲基化的胞嘧啶甲基化的胞嘧啶可以区分。

Tags

遗传学，56期，表观遗传学，DNA甲基化，亚硫酸氢，5 - 甲基胞嘧啶（5 - MEC），PCR
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