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JoVE Journal
Biology

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DNA甲基化:亚硫酸氢修改和分析
 
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DNA甲基化:亚硫酸氢修改和分析

Article DOI: 10.3791/3170
October 21st, 2011

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Summary October 21st, 2011

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DNA甲基化分析的黄金标准是亚硫酸氢转换的DNA基因组测序。 5 - 甲基胞嘧啶(5 - MEC)亚硫酸氢在酸性条件下脱氨相比,这种方法需要利用胞嘧啶的敏感性增加。后PCR扩增目标基因组DNA甲基化的胞嘧啶甲基化的胞嘧啶可以区分。

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