19,309 Views
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09:14 min
January 27, 2016
DOI:
10.3791/53620-v
我々は、高スループットシーケンシング(DamID-SEQ)に、DNAアデニンメチルトランスフェラーゼ識別(DamID)を結合することにより、本明細書のアッセイを説明します。この改良された方法は、より高い解像度、より広いダイナミックレンジを提供し、可能にする等のChIP-seqの、RNA-配列などの他のハイスループットシークエンシングデータと併せてDamID、配列データを分析します
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Wu, F., Olson, B. G., Yao, J. DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments. J. Vis. Exp. (107), e53620, doi:10.3791/53620 (2016).
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