19,334 Views
•
09:14 min
•
January 27, 2016
DOI:
Transcript
Automatically generated
Summary
Automatically generated
Wir beschreiben hier einen Test durch Kopplung DNA-Adenin-Methyltransferase-Identifikation (DAMiD), um Hochdurchsatz-Sequenzierung (DAMiD-seq). Dieses verbesserte Verfahren bietet eine höhere Auflösung und einen größeren Dynamikbereich und erlaubt die Analyse DAMiD-seq Daten in Verbindung mit anderen Hochdurchsatz-Sequenzierung Daten wie ChIP-seq, RNA-seq usw.
Related Videos
10:43
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Related Videos
11022 Views
09:27
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
Related Videos
6168 Views
09:14
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Related Videos
7066 Views
08:40
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Related Videos
4735 Views
11:19
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
Related Videos
15907 Views
10:45
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Related Videos
58287 Views
09:52
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Related Videos
24302 Views
12:53
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
Related Videos
31391 Views
08:07
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Related Videos
7960 Views
10:05
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Related Videos
8218 Views
Read Article
Cite this Article
Wu, F., Olson, B. G., Yao, J. DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments. J. Vis. Exp. (107), e53620, doi:10.3791/53620 (2016).
Copy