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January 27, 2016
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We beschrijven hierin een test door het koppelen van DNA adenine methyltransferase identificatie (damid) tot high throughput sequencing (damid-volgende). Deze verbeterde werkwijze een hogere resolutie en een groter dynamisch bereik en maakt analyse damid-seq data in combinatie met andere high throughput sequentiebepaling gegevens zoals ChIP-seq, RNA-seq, etc.
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Cite this Article
Wu, F., Olson, B. G., Yao, J. DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments. J. Vis. Exp. (107), e53620, doi:10.3791/53620 (2016).
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