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DamID-SEQ：蛋白质-DNA相互作用的全基因组映射腺嘌呤甲基化的DNA片段通过高通量测序
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JoVE Journal Biology
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
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DamID-SEQ：蛋白质-DNA相互作用的全基因组映射腺嘌呤甲基化的DNA片段通过高通量测序

DOI:
10.3791/53620-v

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09:14 min

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January 27, 2016

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, ,
1Department of Cell Biology,Yale University School of Medicine

Chapters

  • 00:05Title
  • 00:58Generation and Expression of Fusion Proteins and Free Dam Proteins
  • 03:12Library Preparation: Fragmentation
  • 05:29Library Preparation: Size Selection
  • 07:26Results: Detecting Lamin B1 Binding Regions on the Mouse Chromosome One
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述这里的一个检测通过结合DNA腺嘌呤甲基识别（DamID），以高通量测序（DamID-SEQ）。这种改进的方法提供了更高的分辨率和更宽的动态范围，并允许在分析与其他高通量测序数据如芯片起，RNA测序等一起DamID-SEQ数据

Tags

DamID-seqGenome-wide MappingProtein-DNA InteractionsHigh Throughput SequencingAdenine-methylated DNA FragmentsNuclear OrganizationTranscription RegulationMammalian CellsGenomic RegionsAmyloidTranscription FactorsHigh ResolutionDNA MicroarraysCross-analysisGene CloningDamID VectorLentiviral StocksLentiviral Expression SystemCotransfectionLentivirus Infection

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