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January 13, 2017
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ونحن نقدم وسيلة لعزل وثقافة قناة وولف الماوس (WD). نحن أيضا يبرهن على وجود إجراءات مفصلة لجبل بأكمله المناعية من WDS مثقف / المعزولة حديثا مع الأجسام المضادة الموسومة fluorescently. معا، وتمكن هذه التقنيات دراسة التطور WD، اللف، والتمايز.
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Cite this Article
Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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