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JoVE Journal Developmental Biology
Órgão Cultura e Whole Mount Imunofluorescência Coloração de rato Wolff Dutos
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Órgão Cultura e Whole Mount Imunofluorescência Coloração de rato Wolff Dutos

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

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Nós apresentamos um método para isolamento e cultura do duto de Wolff rato (WD). Também demonstramos um procedimento detalhado para toda a imunocoloração monte da cultura / WDs recentemente isolados com anticorpos marcados fluorescentes. Em conjunto, estas técnicas permitem o estudo do desenvolvimento WD, de enrolamento, e a diferenciação.

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Biologia do Desenvolvimento Edição 119 epidídimo cultura de órgãos imunocoloração duto de Wolff toda montagem,
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