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January 13, 2017
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We presenteren een methode voor de isolatie en de cultuur van de muis Wolffian kanaal (WD). We hebben ook een gedetailleerde procedure voor de hele berg immunokleuring van gekweekte / vers geïsoleerde WDS met fluorescent gelabelde antilichamen aan te tonen. Samen vormen deze technieken kan de studie van de WD ontwikkeling, oprollen, en differentiatie.
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Cite this Article
Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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