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January 13, 2017
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אנו מציגים שיטה בידוד והתרבות של צינור העכבר Wolffian (WD). אנחנו גם מדגימים נוהל מפורט immunostaining הר שלם של WDS תרבותי / טרי מבודד עם נוגדנים מתויג fluorescently. יחד, טכניקות אלו מאפשרים את חקר התפתחות WD, התפתלות, והבחנה.
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Cite this Article
Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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