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January 13, 2017
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Se presenta un método para el aislamiento y cultivo de conducto de Wolff ratón (WD). También demostramos un procedimiento detallado para toda la inmunotinción monte de WDS cultivadas / recién aisladas con anticuerpos marcados con fluorescencia. En conjunto, estas técnicas permiten el estudio del desarrollo de WD, bobinado, y la diferenciación.
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Cite this Article
Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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