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JoVE Journal Developmental Biology
Organ Kultur og Whole Mount Immunofluorescens Farvning af Mouse Wolffian Kanaler
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Organ Kultur og Whole Mount Immunofluorescens Farvning af Mouse Wolffian Kanaler

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

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Vi præsenterer en fremgangsmåde til isolering og dyrkning af muse Wolffian kanalen (WD). Vi viser også en detaljeret procedure for hele mount immunfarvning af dyrkede / frisk isolerede WDS med fluorescens mærkede antistoffer. Tilsammen udgør disse teknikker gør det muligt at studere WD udvikling, opvikling, og differentiering.

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Developmental Biology epididymis orgel kultur immunfarvning Wolffian kanal hele mount,
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