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器官培养和鼠标沃尔弗导管的全山免疫染色

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Developmental Biology

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JoVE Journal Developmental Biology

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Please note that all translations are automatically generated. Click here for the English version.

器官培养和鼠标沃尔弗导管的全山免疫染色

DOI:

10.3791/55134-v

•

09:16 min

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January 13, 2017

•

Manish Kumar, Pradeep Tanwar

¹Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy,University of Newcastle

Chapters

00:05Title
00:51Mouse Embryonic Gonadal Ridge Isolation
03:06Embryonic Gonadal Ridge Culture
04:38Whole Mount Gonadal Ridge Immunofluorescence
06:42Results: Wolffian Duct Morphogenesis and Surface Marker Expression
07:40Conclusion

Summary

Automatic Translation

我们目前的鼠标沃尔夫管（WD）的分离，培养的方法。我们也表现出与荧光标记的抗体培养/新鲜分离的WDS的整装免疫的详细过程。总之，这些技术使WD的发展，卷取和分化的研究。

Tags

Organ Culture Whole Mount Immunofluorescence Mouse Wolffian Ducts Developmental Biology Organ Shape Structure Function In Vivo Cell Culture Niche Factors Embryo Dissection Urogenital System Wolffian Duct Organ Culture Medium

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