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Divisão celular dos mamíferos em matrizes 3D através de microscopia de reflexão Confocal quantitativa
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1Department of Chemical and Biomolecular Engineering,Johns Hopkins University, 2Johns Hopkins Physical Sciences – Oncology Center,Johns Hopkins University, 3Department of Biomedical Engineering,Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 00:32Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
- 04:00Synchronization of Cells Stably Expressing H2B-mCherry
- 06:02Incorporation of the Synchronized Cells into Collagen I Matrices
- 07:18Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
- 08:46Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
- 09:45Conclusion
Este protocolo eficientemente estuda divisão de células de mamíferos em matrizes de colágeno 3D, integrando a sincronização da divisão celular, monitoramento de eventos de divisão em matrizes 3D usando a técnica de imagem live-célula, microscopia de reflexão confocal tempo-resolvido e análise quantitativa de imagem.
