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Säugetier-Zellteilung in 3D Matrizen über Quantitative konfokale Reflexion Mikroskopie
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
DOI:

10:22 min

November 29, 2017

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Chapters

  • 00:05Title
  • 00:32Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
  • 04:00Synchronization of Cells Stably Expressing H2B-mCherry
  • 06:02Incorporation of the Synchronized Cells into Collagen I Matrices
  • 07:18Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
  • 08:46Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
  • 09:45Conclusion

Summary

Automatic Translation

Dieses Protokoll effizient studiert Säugetier-Zellteilung in 3D Kollagen Matrizen durch die Integration von Synchronisation der Zellteilung, Überwachung der Abteilung Veranstaltungen in 3D Matrizen mit live-Cell imaging Technik, zeitaufgelöste konfokale Reflexion Mikroskopie und quantitative bildgebende Analyse.

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