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Zoogdieren celdeling in 3D-Matrices via reflectie van kwantitatieve confocale microscopie
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
DOI:

10:22 min

November 29, 2017

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Chapters

  • 00:05Title
  • 00:32Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
  • 04:00Synchronization of Cells Stably Expressing H2B-mCherry
  • 06:02Incorporation of the Synchronized Cells into Collagen I Matrices
  • 07:18Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
  • 08:46Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
  • 09:45Conclusion

Summary

Automatic Translation

Dit protocol bestudeert efficiënt zoogdieren celdeling in 3D collageen matrices door de integratie van de synchronisatie van de celdeling, toezicht op divisie gebeurtenissen in 3D-matrices met behulp van live-cel beeldvormende techniek, time-resolved confocal reflectie microscopie en kwantitatieve analyse van beeldvorming.

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