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JoVE Journal
Genetics

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Bronchoalveolar Lavage Exosomes i LPS-induceret septisk lunge skade
 

Bronchoalveolar Lavage Exosomes i LPS-induceret septisk lunge skade

Article doi: 10.3791/57737
May 21st, 2018

Summary May 21st, 2018

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Mus udsættes for intraperitoneal LP'ER udskiller exosomes i bronchopneumoni-alveolære lavage (BAL) væske, som er pakket med miRNAs. Ved hjælp af en fælles kultur system, viser vi, at exosomes udgivet i BAL væske forstyrre udtryk for tight junction proteiner i bronchiale epitel celler og øge udtryk af pro-inflammatoriske cytokiner, at fremhæver lunge skade.

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