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人体器官性心脏切片双电压和钙光学映射的临床前心脏电生理评估
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
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人体器官性心脏切片双电压和钙光学映射的临床前心脏电生理评估

DOI:
10.3791/60781-v

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09:35 min

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June 16, 2020

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, ,
1Department of Biomedical Engineering,The George Washington University

Chapters

  • 00:04Introduction
  • 00:29Optical Mapping System Setup
  • 01:16Slicing Protocol
  • 02:41Static Slice Culture
  • 03:22Optical Mapping
  • 05:01Data Processing
  • 07:24Results: Representative Transmembrane Potential and Calcium Transient Mapping
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了用于临床前药物测试的人体心脏切片切片和培养过程，并详细介绍了光学映射用于同时记录这些切片的跨膜电压和细胞内钙信号的过程。

Tags

Optical MappingCardiac ElectrophysiologyHuman Organotypic Cardiac SlicesVoltage DyeCalcium DyeAction PotentialsIntracellular Calcium TransientsPerfusion SystemVibratomeCardioplegiaTyrode’s Recovery SolutionPreclinical Physiological Drug Testing

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