Table of Contents: Issue 46, December 2010

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Sébastien Degot¹, Muriel Auzan¹, Violaine Chapuis¹, Anne Béghin², Amélie Chadeyras¹, Constantin Nelep¹, Maria Luisa Calvo-Muñoz¹, Joanne Young¹, François Chatelain¹, Alexandra Fuchs¹

¹CYTOO Cell Architects, Grenoble, France, ²Centre Commun de Quantimétrie, Faculté de Médecine Rockefeller, Lyon, France

JoVE 2514

Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs

Shantanu Balkundi*¹, Ari S. Nowacek*¹, Upal Roy¹, Andrea Martinez-Skinner¹, JoEllyn McMillan¹, Howard E. Gendelman¹

¹Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

JoVE 2460

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

Michael Boska¹, Yutong Liu¹, Mariano Uberti¹, Balarininvasa R. Sajja¹, Shantanu Balkundi², JoEllyn McMillan², Howard E. Gendelman²

¹Department of Radiology, University of Nebraska Medical Center, ²Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

JoVE 2459

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

M. Dean Chamberlain¹, Mark J. Butler¹, Ema C. Ciucurel¹, Lindsay E. Fitzpatrick², Omar F. Khan¹, Brendan M. Leung², Chuen Lo¹, Ritesh Patel², Alexandra Velchinskaya², Derek N. Voice², Michael V. Sefton¹

¹Institute of Biomaterials and Biomedical Engineering / Department of Chemical Engineering and Applied Chemistry, University of Toronto, ²Institute of Biomaterials and Biomedical Engineering, University of Toronto

JoVE 2177

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Yvonne Aratyn-Schaus¹, Patrick W. Oakes¹, Jonathan Stricker², Stephen P. Winter³, Margaret L. Gardel^1,2

¹Institute for Biophysical Dynamics, University of Chicago, ²Physics Department - James Franck Institute, University of Chicago, ³Interdisciplinary Scientist Training Program, University of Chicago

JoVE 2173

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation

Caitlin W. Burke¹, Richard J. Price^1,2

¹Department of Biomedical Engineering, University of Virginia, ²Neurological Surgery , University of Virginia

JoVE 2145

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

Sonya Seif-Naraghi¹, Jennifer Singelyn¹, Jessica DeQuach¹, Pamela Schup-Magoffin¹, Karen Christman¹

¹University of California, San Diego

JoVE 2109

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Laura E. Dickinson¹, Sharon Gerecht¹

¹Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University

JoVE 2413

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Christopher Moraes^1,2, Yu Sun^1,2, Craig A. Simmons^1,2,3

¹Department of Mechanical and Industrial Engineering, University of Toronto, ²Institute of Biomaterials and Biomedical Engineering, University of Toronto, ³Faculty of Dentistry, University of Toronto

JoVE 2224

Gastrointestinal Motility Monitor (GIMM)

Jill M. Hoffman¹, Elice M. Brooks¹, Gary M. Mawe¹

¹Department of Anatomy and Neurobiology, The University of Vermont

JoVE 2435

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Tessa R. Davis¹, Adam G. Schrum¹

¹Department of Immunology, College of Medicine, Mayo Clinic

JoVE 2066

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Forum H. Bhatt¹, Constance J. Jeffery¹

¹Department of Biological Sciences, University of Illinois Chicago - UIC

JoVE 2134

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Pauline Chugh*¹, Kristen Tamburro*¹, Dirk P Dittmer¹

¹Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill

JoVE 2210

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Serge Gueroussov¹, Stefan P. Tarnawsky¹, Xianying A. Cui¹, Kohila Mahadevan¹, Alexander F. Palazzo¹

¹Department of Biochemistry, University of Toronto

JoVE 2387

Bioenergetic Profile Experiment using C2C12 Myoblast Cells

David G. Nicholls¹, Victor M. Darley-Usmar², Min Wu³, Per Bo Jensen³, George W. Rogers³, David A. Ferrick³

¹Buck Institute for Age Research, Novato, CA, ²Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham - UAB, ³Seahorse Bioscience, North Billerica, MA

JoVE 2511

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

Alex H. Tuttle*¹, Matthew M. Rankin*¹, Monica Teta¹, Daniel J. Sartori¹, Geneva M. Stein¹, Gina J. Kim¹, Cristina Virgilio¹, Anne Granger¹, Di Zhou¹, Simon H. Long¹, Alisa B. Schiffman¹, Jake A. Kushner¹

¹Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine

JoVE 2166

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Ismael C. Gomes*¹, Mariana Acquarone*¹, Renata de Moraes Maciel*¹, Rafael Bierig Erlich¹, Stevens K. Rehen¹

¹Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil

JoVE 2344

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Gary Balian¹, Gina Beck¹, Vedavathi Madhu¹, Robert Sikes², Quanjun Cui³, Haixiang Liang¹, Joshua Bush¹

¹Orthopaedics Research, University of Virginia, ²Biological Sciences, University of Delaware, ³Orthopaedic Surgery, University of Virginia

JoVE 2362

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

Magdalena Sobczak¹, Jillian Dargatz¹, Magdalena Chrzanowska-Wodnicka¹

¹Blood Research Institute, BloodCenter of Wisconsin

JoVE 2316

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

Usheer Kanjee¹, Walid A. Houry¹

¹Department of Biochemistry, University of Toronto

JoVE 2094

Profiling of Methyltransferases and Other S-adenosyl-_L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

Thomas Lenz¹, Peter Poot², Olivia Gräbner¹, Mirko Glinski¹, Elmar Weinhold², Mathias Dreger¹, Hubert Köster¹

¹Department of Biochemistry / Analytics, caprotec bioanalytics GmbH, ²Institute of Organic Chemistry, RWTH Aachen University

JoVE 2264

Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes

Pratima Thakur¹, Alla F. Fomina¹

¹Department of Physiology and Membrance Biology, University of California, Davis

JoVE 2346

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

H. Arthur Jeiranian*¹, Brandy J. Schalow*¹, Justin Courcelle¹

¹Department of Biology, Portland State University

JoVE 2220

Microinjection of Medaka Embryos for use as a Model Genetic Organism

Sean R. Porazinski¹, Huijia Wang¹, Makoto Furutani-Seiki¹

¹Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath

JoVE 1937

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Sean R. Porazinski¹, Huijia Wang¹, Makoto Furutani-Seiki¹

¹Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath

JoVE 2055

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Niva Russek-Blum*¹, Helit Nabel-Rosen*¹, Gil Levkowitz¹

¹Molecular Cell Biology, Weizmann Institute of Science

JoVE 1902

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

Mariya M. Kucherenko¹, April K. Marrone¹, Valentyna M. Rishko¹, Andriy S. Yatsenko¹, Annekatrin Klepzig¹, Halyna R. Shcherbata¹

¹Gene Expression and Signaling Research Group, Max Planck Institute for Biophysical Chemistry

JoVE 2438

Isolation of Valvular Endothelial Cells

Russell A. Gould¹, Jonathan T. Butcher¹

¹Department of Biomedical Engineering, Cornell University

JoVE 2158

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

Claudia Husseneder*¹, Amit Sethi*¹, Lane Foil¹, Jennifer Delatte¹

¹Department of Entomology, Louisiana State University Agricultural Center

JoVE 2190

Bioassays for Monitoring Insecticide Resistance

Audra L.E. Miller¹, Kelly Tindall¹, B. Rogers Leonard²

¹Division of Plant Sciences, University of Missouri, Delta Research Center, ²Department of Entomology, Louisiana State University Agricultural Center

JoVE 2129

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Jijun Hao¹, Charles H. Williams¹, Morgan E. Webb¹, Charles C. Hong^1,2,3,4

¹Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, ²Department of Pharmacology, Vanderbilt University School of Medicine, ³Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, ⁴Research Medicine, Veterans Affairs TVHS, Vanderbilt University School of Medicine

JoVE 2243

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Baomei Wang¹, Bernd H. Zinselmeyer^1,2, Jeremiah R. McDole¹, Peggy A. Gieselman¹, Mark J. Miller¹

¹Department of Pathology and Immunology, Washington University in St. Louis, ²National Institute of Neurological Disorders and Stroke, NINDS, NIH - National Institute of Health

JoVE 2062

Development of a Negative Selectable Marker for Entamoeba histolytica

Mayuresh M Abhyankar¹, Sarah M Haviland¹, Carol A Gilchrist¹, William A Petri, Jr.¹

¹Division of Infectious Disease and International Health, University of Virginia Health System

JoVE 2410

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

Shey-Cherng Tzou¹, Melissa A. Landek-Salgado¹, Hiroaki Kimura¹, Patrizio Caturegli¹

¹Department of Pathology, The Johns Hopkins University

JoVE 2181

Induction of Experimental Autoimmune Hypophysitis in SJL Mice

Melissa A. Landek-Salgado¹, Shey-Cherng Tzou¹, Hiroaki Kimura¹, Patrizio Caturegli¹

¹Department of Pathology, The Johns Hopkins University

JoVE 2182

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

Nayan J. Sarma¹, Akiko Takeda¹, Nabeel R. Yaseen¹

¹Department of Pathology and Immunology, Washington University School of Medicine

JoVE 2195

Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis

Tobias Traeger*¹, Pia Koerner*¹, Wolfram Kessler¹, Katharina Cziupka¹, Stephan Diedrich¹, Alexandra Busemann¹, Claus-Dieter Heidecke¹, Stefan Maier¹

¹Department of Surgery, University of Greifswald

JoVE 2299

Real-time Imaging of Leukotriene B₄ Mediated Cell Migration and BLT1 Interactions with β-arrestin

Venkatakrishna R. Jala¹, Bodduluri Haribabu¹

¹Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville

JoVE 2315

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Nazzy Pakpour¹, Kong Wai Cheung¹, Lattha Souvannaseng¹, Jean-Paul Concordet², Shirley Luckhart¹

¹Department of Medical Microbiology and Immunology, University of California, Davis, ²Département Génétique et Développement, Institut Cochin, Université Paris Descartes

JoVE 2355

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

Kelly Schwartz¹, Rachel Stephenson¹, Margarita Hernandez¹, Nicolays Jambang¹, Blaise R. Boles¹

¹Molecular, Cellular, and Developmental Biology, University of Michigan

JoVE 2470

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Rachel A. McGovern¹, P. Richard Harrigan¹, Luke C. Swenson¹

¹Laboratory Program, BC Centre for Excellence in HIV/AIDS

JoVE 2531

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Mandy Stubbendorff¹, Tobias Deuse^1,2, Anna Hammel¹, Robert C. Robbins², Hermann Reichenspurner¹, Sonja Schrepfer^1,2

¹University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, ²Stanford University School of Medicine

JoVE 1989

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Ronald X. Xu¹, Kun Huang², Ruogu Qin¹, Jiwei Huang¹, Jeff S. Xu¹, Liya Ding², Urmila S. Gnyawali³, Gayle M. Gordillo³, Surya C. Gnyawali^3,4, Chandan K. Sen³

¹Department of Biomedical Engineering, The Ohio State University, ²Department of Biomedical Informatics, The Ohio State University, ³Comprehensive Wound Center, The Ohio State University, ⁴Department of Surgery, The Ohio State University

JoVE 2095

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

Simon Rajendran¹, Slawomir Salwa¹, Xuefeng Gao², Sabin Tabirca², Deirdre O'Hanlon², Gerald C. O'Sullivan¹, Mark Tangney¹

¹Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, ²Department of Computer Science, University College Cork

JoVE 2378

Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

Oscar Gonzalez-Perez¹, Hugo Guerrero-Cazares², Alfredo Quiñones-Hinojosa²

¹ Neuroscience Lab/ Fac. Psicologia, University of Colima, ²Department of Neurosurgery, Johns Hopkins University

JoVE 2082

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

Tadeusz J. Kaczynski¹, Shermali Gunawardena¹

¹Department of Biological Sciences, SUNY-University at Buffalo

JoVE 2150

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Benoit V. Jacquet¹, Philip Ruckart¹, H. Troy Ghashghaei¹

¹Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University

JoVE 2486

One Dimensional Turing-Like Handshake Test for Motor Intelligence

Amir Karniel¹, Guy Avraham¹, Bat-Chen Peles¹, Shelly Levy-Tzedek¹, Ilana Nisky¹

¹Biomedical Engineering, Ben-Gurion University

JoVE 2492

A Novel Method for Assessing Proximal and Distal Forelimb Function in the Rat: the Irvine, Beatties and Bresnahan (IBB) Forelimb Scale

Karen-Amanda Irvine¹, Adam R. Ferguson¹, Kathleen D. Mitchell¹, Stephanie B. Beattie¹, Michael S. Beattie¹, Jacqueline C. Bresnahan¹

¹Department of Neurological Surgery, University of California, San Francisco

JoVE 2246

Combining Computer Game-Based Behavioural Experiments With High-Density EEG and Infrared Gaze Tracking

Keith J. Yoder^1,2, Matthew K. Belmonte^1,3

¹Department of Human Development, Cornell University, ²Social Sciences Division, University of Chicago, ³National Brain Research Centre, Manesar, India

JoVE 2320

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Sophie Imbeault*¹, Nicolas Valenzuela*², Stephen Fai², Steffany A.L. Bennett¹

¹Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ²Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism, Carleton University

JoVE 2483

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

Collin J Lobb¹, Carlos A Paladini¹

¹Neurosciences Institute, University of Texas San Antonio - UTSA

JoVE 2275

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network

Mark A. Halko¹, Mark C. Eldaief¹, Jared C. Horvath¹, Alvaro Pascual-Leone¹

¹Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center

JoVE 2271

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism

Lindsay M. Oberman¹, Jared C. Horvath¹, Alvaro Pascual-Leone¹

¹Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center

JoVE 2272

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior

Umer Najib¹, Jared C. Horvath¹, Juha Silvanto², Alvaro Pascual-Leone¹

¹Berenson-Allen Center for Noninvasive Brain Stimulation, Beth Israel Deaconess Medical Center, ²Brain Research Unit, Low Temperature Laboratory and Advanced magnetic Imaging Center, Aalto University School of Science and Technology

JoVE 2273

Erratum: Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Erratum: Performing and Processing FNA of Anterior Fat Pad for Amyloid