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Table of Contents

Table of Contents: Issue 132, February 2018

Jump to:  Editorial (1) Biology (7) Neuroscience (14) Immunology and Infection (8) Medicine (17) Bioengineering (8) Engineering (6) Chemistry (11) Environment (2) Behavior (4) Developmental Biology (9) Genetics (4) Biochemistry (6) Cancer Research (5)

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

1, 1, 2,3, 1, 2,3, *1, *1,4

1Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, 2Department of Microbiology and Immunology, Albert Einstein College of Medicine, 3Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, 4Faculty of Ceilândia, University of Brasília

JoVE 57127

 Biology

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

1, 1, 2, 2, 3,4, 3, 1, 1, 1, 5, 5, 4,6, 1

1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG

JoVE 56580

 Biology

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

1, 1, 1,2, 1, 1

1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center, Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute, University of Cambridge

JoVE 56904

 Neuroscience

Online Transcranial Magnetic Stimulation Protocol for Measuring Cortical Physiology Associated with Response Inhibition

1, 2, 2, 2,3, 2, 4, 2

1College of Medicine, University of Cincinnati, 2Division of Neurology, Cincinnati Children's Hospital Medical Center, 3Division of Psychiatry, Cincinnati Children's Hospital Medical Center, 4Center for Neurodevelopmental and Imaging Research, Kennedy Krieger Institute

JoVE 56789

 Neuroscience

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

*1, *1,2, 1,2, 1,2,3, 1,2, 4

1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Mechanical Engineering, Stanford University, 3Department of Bioengineering, Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO)

JoVE 56530

 Neuroscience

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

1,2,3, 1,2,3,4, 4, 1,2,3,4, 1,2,3,4

1Transplant and Stem Cell Immunobiology Lab, University Heart Center, 2Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF), 3Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research, 4Cardiovascular Surgery, University Heart Center

JoVE 56477

 Medicine

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report

1, 2, 1, 3, 4

1Low Vision Research Centre of Milan, 2Department of Ophthalmology, A. Fiorini Hospital, Sapienza University of Rome, 3Glaucoma and Low Vision Study Center, Department of General Surgery and Organ Transplants, University of Bologna, 4Department of Sense Organs, Faculty of Medicine and Odontology, Sapienza University of Rome

JoVE 56469

 Medicine

An Anatomical Study of Nerves at Risk During Minimally Invasive Hallux Valgus Surgery

1,2,3, 1,4, 1,5, 6, 1

1Laboratory of Arthroscopic and Surgical Anatomy. Department of Pathology and Experimental Therapeutics (Human Anatomy and Embryology Unit), University of Barcelona, 2Health Sciences Faculty of Manresa, University of Vic-Central University of Catalunya, 3Groupe de Recherche et d'Etude en Chirurgie Mini-Invasive du Pied, GRECMIP, 4Foot and Ankle Unit, Hospital Quirón Barcelona, 5Foot and Ankle Unit, Orthopedic and Trauma Surgery, Royal London Hospital, Barts Health NHS Trust, 6Department of Orthopedic Surgery, Foot and Ankle Unit, University of Minnesota

JoVE 56232

 Medicine

Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening

*1, *1, *1,2,3, 2,3, 1, 1, 1, 1, 1, 1

1Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, 2Center for Translational Medicine, Nanjing University Medical School, 3Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School

JoVE 56276

 Medicine

In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Menière's Disease Ears and Normal Hearing Ears

1, 2, 1, 3, 1

1Department of Radiology, University Hospital, LMU Munich, 2Department of Otorhinolaryngology - Head and Neck Surgery, SLK-Kliniken Heilbronn GmbH, 3Department of Otorhinolaryngology Head and Neck Surgery, Ludwig-Maximilians-University Hospital Munich, German Centre for Vertigo and Balance Disorder

JoVE 57091

 Medicine

A Surgical Procedure for the Administration of Drugs to the Inner Ear in a Non-Human Primate Common Marmoset (Callithrix jacchus)

*1,2, *3, *1,2, 4, 3, 2, 1

1Division of Regenerative Medicine, Jikei University School of Medicine, 2Department of Otorhinolaryngology, Jikei University School of Medicine, 3Department of Otorhinolaryngology, Head and Neck Surgery, Keio University School of Medicine, 4Laboratory Animal Facilities, Jikei University School of Medicine

JoVE 56574

 Medicine

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

*1, *1, 2, 1, 1, 1, 1, 3, 1, 4

1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group

JoVE 55414

 Bioengineering

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

*1, *1, 1,2, 3,4, 1, 1, 1, 2, 2, 5, 6, 7,8

1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University

JoVE 57314

 Bioengineering

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

1, 1, 1,2, 3, 1,2

1Department Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, 2Translational Center Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research ISC, 3Fujifilm Manufacturing Europe B.V.

JoVE 57363

 Bioengineering

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

1, 1, 1, 1, 2, 2, 3, 1

1Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 2Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, 3TissUse GmbH

JoVE 56412

 Bioengineering

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

1, 2,3, 1, 2,4, 1,2

1Kellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, 4Department of Radiology, University of Michigan

JoVE 57135

 Engineering

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

1, 1, 1, 1, 2, 1, 3, 1,4, 4

1Institute of Fluid Mechanics, FAU Busan Campus, University of Erlangen-Nuremberg, 2Institute of Chemical Reaction Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 3Institute of Bioprocess Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 4Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

JoVE 57238

 Engineering

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

1, 2, 2, 3, 2, 4

1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

JoVE 57169

 Chemistry

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 1,2

1Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, 2Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux

JoVE 55041

 Chemistry

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3,4, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3

1Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, 2Institute of Materials Engineering, College of Engineering and Applied Sciences, Nanjing University, 3Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, 4Department of Chemistry, Western Washington University

JoVE 56657

 Chemistry

Paradigms of Lower Extremity Electrical Stimulation Training After Spinal Cord Injury

1,2, 1, 1, 3, 4

1Spinal Cord Injury and Disorders Service, Hunter Holmes McGuire VAMC, 2Department of Physical Medicine and Rehabilitation, Virginia Commonwealth University, 3Deceased, Department of Kinesiology, The University of Georgia, 4Department of Physical Medicine and Rehabilitation, Penn State Milton S. Hershey Medical Center

JoVE 57000

 Behavior

An Objective and Child-friendly Assessment of Arm Function by Using a 3-D Sensor

1, 1, 2, 3, 4,5, 4,5, 6, 1

1Data Science, Roche Pharmaceutical Research and Early Development Informatics, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 2Translational Technologies and Bioinformatics, Pharmaceutical Sciences, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 3Biomarker Experimental Medicine, Neuroscience, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 4Division of Neuropediatrics, University of Basel Children's Hospital, 5Department of Neurology, University of Basel Hospital, 6Translational Medicine, Neuroscience and Rare Diseases, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd.

JoVE 57014

 Behavior

A New Method for Inducing a Depression-Like Behavior in Rats

*1, *2, 2, 2, 3, 2, 2, 2, 2, 2

1Department of Emergent Medicine, Soroka University Medical Center, Ben-Gurion University of the Negev, 2Division of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 3Department of Biophysics and Biochemistry, Oles' Honchar Dnipro National University

JoVE 57137

 Behavior

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

*1,2, *1, 1, 1, 3, 3, 3, 2,3, 1,2, *3, *1

1Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health, Università degli Studi di Milano, 3Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS

JoVE 57263

 Developmental Biology

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

1, 2, 2,3

1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

JoVE 56945

 Cancer Research

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

1, 2,3,4, 2,3, 2,3, 2,3,5, 6, 7, 2, 2, 2, 2,3,6, 8, 2,3, 4, 2,6, *4, *2,4

1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou

JoVE 56606

 Cancer Research

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2

1Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine, University of Utah, 2Huntsman Cancer Institute, University of Utah

JoVE 57182

 Cancer Research

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