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Table of Contents

Table of Contents: Issue 146, April 2019

Jump to:  Editorial (1) Behavior (8) Biochemistry (6) Bioengineering (8) Biology (3) Cancer Research (7) Chemistry (7) Developmental Biology (10) Engineering (6) Environment (3) Genetics (8) Immunology and Infection (19) Medicine (15) Neuroscience (11) Errata (5)

An Instrumented Pull Test to Characterize Postural Responses

1Department of Medical Bionics, The University of Melbourne, 2Department of Neurology, The Royal Melbourne Hospital, 3Department of Neurology, Austin Hospital, 4The Bionics Institute, 5Department of Medicine, The University of Melbourne, 6Department of Physiotherapy, The University of Melbourne, 7Medical Research Council Brain Network Dynamics Unit, University of Oxford

JoVE 59309

 Behavior

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

JoVE 58669

 Biochemistry

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital

JoVE 59173

 Bioengineering

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

1Department of Radiation Oncology, University of Colorado Denver - Anschutz Medical Campus, 2Department of Anesthesiology, University of Colorado Denver - Anschutz Medical Campus, 3Division of Radiology, University of Colorado Denver - Anschutz Medical Campus

JoVE 59195

 Cancer Research

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University

JoVE 59507

 Cancer Research

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

1Institut des matériaux, École Polytechnique Fédérale de Lausanne (EPFL), 2Laboratory for Functional Polymers, Swiss Federal Laboratories for Materials Science and Technology (Empa), 3Faculty of Engineering and Natural Sciences, Sabanci University, 4SUNUM Nanotechnology Research Centre, Sabanci University

JoVE 58883

 Chemistry

Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

1Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 2Department for Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, 3Ludwig Boltzmann Institute Applied Diagnostics, 4CBmed GmbH - Center for Biomarker Research in Medicine

JoVE 59557

 Chemistry

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne

JoVE 59313

 Developmental Biology

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

JoVE 59297

 Developmental Biology

Neurogenesis Using P19 Embryonal Carcinoma Cells

1Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 2Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding, Polish Academy of Sciences

JoVE 58225

 Developmental Biology

Radio Frequency Magnetron Sputtering of GdBa2Cu3O7δ/ La0.67Sr0.33MnO3 Quasi-bilayer Films on SrTiO3 (STO) Single-crystal Substrates

1Department of Physics, Mathematics, Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, 2Key Laboratory of Artificial Structure and Quantum Control, Ministry of Education, Department of Physics, Shanghai Jiao Tong University, 3Shanghai Institute of Space Power-sources, 4Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University

JoVE 58069

 Engineering

Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster (Nephrops norvegicus)

1Universitat Oberta de Catalunya, 2Institute of Marine Sciences, Spanish National Research Council (CSIC), 3Institute for Environmental Protection and Research (ISPRA), 4Department of Biology and Ecology of Fishes, Leibniz-Institute of Freshwater Ecology and Inland Fisheries

JoVE 58515

 Environment

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

JoVE 57289

 Genetics

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

1Department of Developmental Physiology, Centre for Organismal Studies (COS) Heidelberg, 2Department of Cell Biology, Centre for Organismal Studies (COS) Heidelberg, 3Department of Stem Cell Biology, Centre for Organismal Studies (COS) Heidelberg

JoVE 59394

 Genetics

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

JoVE 59218

 Immunology and Infection

Generation of Monoclonal Antibodies Against Natural Products

1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

JoVE 57116

 Immunology and Infection

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux

JoVE 58415

 Immunology and Infection

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

1Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology, McGill Univ.

JoVE 59314

 Immunology and Infection

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

1Infectious Diseases Group, Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering, National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore

JoVE 58829

 Immunology and Infection

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

1Department of Endocrinology & Metabolism Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin

JoVE 59470

 Immunology and Infection

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, 3Center of Infectious Disease and Signaling Research, National Cheng Kung University

JoVE 59132

 Immunology and Infection

Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin

1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII

JoVE 58922

 Immunology and Infection

Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test

1Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Knoxville, TN, United States Department of Agriculture (USDA), 2Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Sutton, MA, United States Department of Agriculture (USDA), 3Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Milton, FL, United States Department of Agriculture (USDA), 4Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Concord, NH, United States Department of Agriculture (USDA), 5Lyssa LLC

JoVE 59416

 Immunology and Infection

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

1Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2National Cancer Institute, National Institutes of Health, 3Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School

JoVE 58469

 Medicine

Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

1Christine M. Kleinert Institute for Hand and Microsurgery, 2Cardiovascular Innovation Institute, University of Louisville, 3Kentucky Spinal Cord Injury Research Center, University of Louisville, 4Department of Physiology, University of Louisville

JoVE 59458

 Medicine

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

1Division of Cardiovascular Surgery, Peter Munk Cardiac Center, Toronto General Hospital, University Health Network, 2Institute of Medical Science, University of Toronto, 3Perfusion and Anesthesia Services, Toronto General Hospital, University Health Network, 4Department of Anesthesia and Pain Management, Toronto General Hospital, University Health Network, 5Department of Anesthesia, University of Toronto, 6Department of Surgery, Faculty of Medicine, University of Toronto

JoVE 59197

 Medicine

Studying Brain Function in Children Using Magnetoencephalography

1ARC Centre of Excellence in Cognition and its Disorders, Macquarie University, 2Department of Cognitive Science, Macquarie University, 3Aston Brain Centre, School of Life and Health Sciences, Aston University, 4Department of Linguistics, Macquarie University

JoVE 58909

 Neuroscience

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS

JoVE 59220

 Neuroscience

An Unbiased Approach of Sampling TEM Sections in Neuroscience

1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, 3Division of General Neurology, Department of Neurology, Medical University of Graz, 4Institute of Pathology, Medical University of Graz, 5Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg

JoVE 58745

 Neuroscience

Real-time Video Projection in an MRI for Characterization of Neural Correlates Associated with Mirror Therapy for Phantom Limb Pain

1Laboratory of Neuromodulation & Center for Clinical Research Learning, Department of Physical Medicine and Rehabilitation, Harvard Medical School, Spaulding Rehabilitation Hospital, 2University of Chicago Medical Center, Department of Neurology, University of Chicago, 3Department of Neuroscience and Behavior, Psychology Institute, University of Sao Paulo, 4The Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 5Center for Biomedical Imaging, Department of Anatomy and Neurobiology, Boston University School of Medicine, 6Spaulding Rehabilitation Hospital, Harvard Medical School, 7Department of Psychology & Milan Center for Neuroscience, University of Milano-Bicocca, 8Neuropsychological Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Auxologico Italiano

JoVE 58800

 Neuroscience

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

1Department of Molecular Genetics and Microbiology, Duke University Medical Center, 2Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 3Department of Neurobiology, Duke University Medical Center, 4Department of Mechanical Systems, Engineering, Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences, Duke University

JoVE 59446

 Neuroscience

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