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Table of Contents

Table of Contents: Issue 160, June 2020

Jump to:  Behavior (6) Biochemistry (10) Bioengineering (9) Biology (24) Cancer Research (6) Chemistry (4) Developmental Biology (6) Engineering (6) Environment (8) Genetics (8) Immunology and Infection (17) Medicine (20) Neuroscience (15) Errata (1)

Non-Radioactive In Vitro Cardiac Myosin Light Chain Kinase Assays

1Department of Medical Biochemistry, Osaka University Graduate School of Medicine/Frontier Biosciences, 2Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine/Frontier Biosciences, 3Department of Cardiovascular and internal medicine, Kanazawa University Graduate School of Medicine


JoVE 61168
 Biochemistry

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences


JoVE 61489
 Biochemistry

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)


JoVE 61440
 Biochemistry

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

1Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, 2Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, 3Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Developmental Biology Center, National Institutes of Health, 4Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute, University of Louisville, 5Department of Pediatrics, School of Medicine, University of Louisville, 6Department of Cardiovascular Surgery, Mitsubishi Kyoto Hospital, 7Cincinnati Children's Heart Institute


JoVE 61246
 Bioengineering

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA


JoVE 61143
 Bioengineering

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht


JoVE 60709
 Biology

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine


JoVE 61371
 Biology

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio


JoVE 61138
 Biology

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital


JoVE 61455
 Biology

Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging

1The Comprehensive Cancer Center, The Ohio State University, 2Department of Radiation Oncology, The Ohio State University, 3Department of Veterinary Biosciences, The Ohio State University, 4Davis Heart & Lung Research Institute, The Ohio State University


JoVE 61272
 Cancer Research

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST)


JoVE 61285
 Cancer Research

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

1Center for Childhood Cancer and Blood Diseases, Abigail Wexner Research Institute at Nationwide Children's Hospital, 2Molecular, Cellular, and Developmental Biology Program, The Ohio State University, 3Division of Pediatric Hematology/Oncology/Blood & Marrow Transplant, The Ohio State University, 4Department of Pediatrics, The Ohio State University


JoVE 61564
 Cancer Research

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias, Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación, Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture, Universidade de Santiago de Compostela

JoVE 60842
 Chemistry

An Externally-Heated Diamond Anvil Cell for Synthesis and Single-Crystal Elasticity Determination of Ice-VII at High Pressure-Temperature Conditions

1Gemmological Institute, China University of Geosciences (Wuhan), 2Hawai'i Institute of Geophysics and Planetology, University of Hawai'i at Mānoa, 3Department of Earth and Planetary Sciences, University of New Mexico, 4Center for Advanced Radiation Sources, University of Chicago


JoVE 61389
 Chemistry

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

1SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

JoVE 60945
 Chemistry

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), 3Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora-UMA-CSIC)

JoVE 60241
 Developmental Biology

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery, University of North Carolina at Chapel Hill


JoVE 61242
 Developmental Biology

Measurement of Chladni Mode Shapes with an Optical Lever Method

1School of Materials Science and Engineering, University of Science and Technology Beijing, 2School of Civil and Resource Engineering, University of Science and Technology Beijing, 3Basic Experimental Center for Natural Science, University of Science and Technology Beijing, 4School of Mathematics and Physics, University of Science and Technology Beijing


JoVE 61020
 Engineering

Graphene-Assisted Quasi-van der Waals Epitaxy of AlN Film on Nano-Patterned Sapphire Substrate for Ultraviolet Light Emitting Diodes

1State Key Laboratory of Solid-State Lighting, Institute of Semiconductors, Chinese Academy of Sciences, 2Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, 3Center for Nanochemistry (CNC), Beijing National Laboratory for Molecular Science, College of Chemistry and Molecular Engineering, Peking University, 4State Key Laboratory of Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences, 5Electron Microscopy Laboratory, School of Physics, Peking University

JoVE 60167
 Engineering

Ground-level Unmanned Aerial System Imagery Coupled with Spatially Balanced Sampling and Route Optimization to Monitor Rangeland Vegetation

1Wyoming Reclamation and Restoration Center, University of Wyoming, 2Department of Ecosystem Science and Management, University of Wyoming, 3Program in Ecology, University of Wyoming, 4Wyoming Geographic Information Science Center, University of Wyoming, 5Wyoming State Office, Bureau of Land Management, 6School of Mathematics and Statistics, University of Canterbury, 7Department of Statistics, University of Wyoming


JoVE 61052
 Environment

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University


JoVE 61478
 Environment

Simulating Impacts of Ice Storms on Forest Ecosystems

1Northern Research Station, U.S. Forest Service, Durham, NH, 2Department of Civil and Environmental Engineering, Syracuse University, 3Northern Research Station, U.S. Forest Service, North Woodstock, NH, 4Department of Natural Resources, Cornell University, 5Department of Civil and Environmental Engineering, Southern Illinois University, 6Advanced Science Research Center at the Graduate Center, City University of New York, 7Cary Institute of Ecosystem Studies, 8Rubenstein School of Environment and Natural Resources, University of Vermont, 9Department of Forestry and Natural Resources, University of Kentucky, 10Northern Research Station, U.S. Forest Service, Burlington, VT


JoVE 61492
 Environment

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications


JoVE 61414
 Immunology and Infection

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin

JoVE 60914
 Immunology and Infection

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital


JoVE 61069
 Immunology and Infection

Analysis of Human Natural Killer Cell Metabolism

1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health


JoVE 61466
 Immunology and Infection

Optimization of the Cuff Technique for Murine Heart Transplantation

1Organ Transplantation Institute, School of Medicine, Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center, Central South University, 6School of Medicine, Xiamen University, 7Xiamen Medical College, 8Medical College, Guangxi University

JoVE 61103
 Immunology and Infection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 2School of Life Science, University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences


JoVE 60523
 Immunology and Infection

Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 2Environment and Sustainability Institute, University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5Direction des Services Vétérinaires, 6Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, 7Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, 8FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, 9Swiss Tropical and Public Health Institute, 10University of Basel, 11Institut National de Recherche Biomédicale

JoVE 60008
 Immunology and Infection

Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video

1Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 2Department of Surgery, Southampton University Hospital NHS Foundation Trust, 3General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona

JoVE 60332
 Medicine

External Cephalic Version: Is it an Effective and Safe Procedure?

1Department of Obstetrics & Gynecology, "Virgen de la Arrixaca", University Clinical Hospital, 2Department of Gynecology, "Reina Sofía", University Clinical Hospital, 3Department of Obstetrics and Gynecology, Pediatrics and Surgery, University of Murcia, 4Institute for Biomedical Research of Murcia

JoVE 60636
 Medicine

Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System

1Ludwig-Boltzmann-Institute for Cardiovascular Research at the Center for Biomedical Research, Medical University of Vienna, 2Concept & Ideation, Electrical Sector/EMEA/Power Distribution Division, Eaton Industries (Austria) GmbH, 3Vetfarm and Clinical Unit for Herd Health Management in Ruminants, Clinic for Ruminants, Vetmeduni Vienna

JoVE 61033
 Medicine

Noninvasive Electrocardiography in the Perinatal Mouse

1Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, 2Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England. Biddeford, ME, 3College of Arts and Sciences, University of New England, Biddeford, ME, 4Department of Pediatrics, Division of Pediatric Cardiology, Maine Medical Center, Portland, ME


JoVE 61074
 Medicine

Using a Chemical Biopsy for Graft Quality Assessment

1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, 2Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, 3Department of Transplantation and General Surgery, University Hospital, University of Nicolaus Copernicus Torun, 4Department of Medicine, Toronto General Hospital


JoVE 60946
 Medicine

Evaluation of Color Difference in Placenta with Twin Anemia Polycythemia Sequence

1Department of Reproductive Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, 2Division of Fetal Medicine, Department of Obstetrics, Leiden University Medical Center, 3Fetal Medicine Unit & Prenatal Diagnosis Center, Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, 4Division of Neonatology, Department of Pediatrics, Leiden University Medical Center


JoVE 61312
 Medicine

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

1Division of Neonatology, Stead Family Department of Pediatrics, University of Iowa, 2Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Iowa, 3Division of Neonatology, Department of Pediatrics, Vanderbilt University, 4Department of Microbiology & Immunology, University of Iowa


JoVE 61464
 Medicine

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains

1Department of Neurology and Neuropathology, Golgi-Cenci Foundation, 2Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, 3Department of Neuropsychology and Social Sciences, Golgi-Cenci Foundation, 4Department of Rehabilitation, ASP Golgi-Redaelli Geriatric Hospital, 5Genomic and Post-Genomic Center, IRCCS Mondino Foundation, 6Department of Brain and Behavioral Sciences, University of Pavia, 7Department of Pathology, ASP Golgi-Redaelli Geriatric Hospital, 8Department of Neurological Science, IRCCS Mondino Foundation, University of Pavia


JoVE 60296
 Neuroscience

Use of Real-Time Functional Magnetic Resonance Imaging-Based Neurofeedback to Downregulate Insular Cortex in Nicotine-Addicted Smokers

1Departamento de Psiquiatría, Escuela de Medicina, Centro Interdisciplinario de Neurociencias, Pontificia Universidad Católica de Chile, 2Laboratory for Brain-Machine Interfaces and Neuromodulation, Pontificia Universidad Católica de Chile, 3Institute of Medical Psychology and Behavioral Neurobiology, University of Tübingen, 4Department of Psychiatry and Psychotherapy, University of Tübingen, 5Unidad de Imágenes Cuantitativas Avanzadas, Departamento de Imaágenes, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, 6División de Neurociencia, Centro de Investigación en Complejidad Social (neuroCICS), Facultad de Gobierno, Universidad del Desarrollo, 7Institute di Ricovero e Cura a Carattere Scientifico, 8Wyss Center for Bio and Neuroengineering, 9Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile


JoVE 59441
 Neuroscience

Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles

1Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, 2Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, 3Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, 4Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología


JoVE 61402
 Neuroscience

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