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Table of Contents

Table of Contents: Issue 135, May 2018

Jump to:  Editorial (1) Behavior (6) Biochemistry (6) Bioengineering (17) Biology (8) Cancer Research (8) Chemistry (6) Developmental Biology (6) Engineering (11) Environment (6) Genetics (14) Immunology and Infection (16) Medicine (19) Neuroscience (18) Errata (1)

Tickling, a Technique for Inducing Positive Affect When Handling Rats

1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University, 2Canadian Council on Animal Care, 3Department of Animal Sciences, Center for Animal Welfare Science, College of Agriculture, Purdue University, 4Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

JoVE 57190

 Behavior

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, 3School of Materials Science and Chemical Engineering, Ningbo University

JoVE 57592

 Biochemistry

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute, University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute, University of Calgary

JoVE 57440

 Bioengineering

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

JoVE 57166

 Bioengineering

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen

JoVE 57551

 Bioengineering

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University

JoVE 57384

 Bioengineering

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

1Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, 2Angiocrine Biosciences, Inc., 3Tri-Institutional Stem Cell Derivation Laboratory, Weill Cornell Medical College, 4Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medical College

JoVE 57472

 Bioengineering

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions

1Engineering Laboratory for Functionalized Carbon Materials and Shenzhen Key Laboratory for Graphene-based Materials, Graduate School at Shenzhen, Tsinghua University, 2School of Materials Science and Engineering, Tsinghua University, 3Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 4Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, 5School of Chemical Engineering and Technology, Tianjin University

JoVE 57144

 Bioengineering

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

JoVE 57712

 Bioengineering

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

1Department of Surgery, University of Wisconsin-Madison, 2Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, 3Department of Biomedical Engineering, University of Wisconsin College of Engineering, 4Department of Biomedical Engineering, Worcester Polytechnic Institute

JoVE 57586

 Bioengineering

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg

JoVE 56394

 Cancer Research

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

1Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine, The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School

JoVE 57558

 Cancer Research

Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode

1State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, 2Department of Mathematics, Quaid-I-Azam University, 3NAAM Research Group, Faculty of Science, King Abdulaziz University

JoVE 57260

 Engineering

Tracking Infiltration Front Depth Using Time-lapse Multi-offset Gathers Collected with Array Antenna Ground Penetrating Radar

1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 2National Institute for Rural Engineering, National Agriculture and Food Research Organization, 3Arid Land Research Center, Tottori University, 4GeoFive Co. Ltd, 53D-Radar

JoVE 56847

 Environment

Stress Distribution During Cold Compression of Rocks and Mineral Aggregates Using Synchrotron-based X-Ray Diffraction

1Mineral Physics Institute, Department of Geoscience, Stony Brook University, 2Geological Engineering, Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 3Rock and Ice Physics Laboratory, Department of Earth Sciences, University College London, 4Department of Chemistry, Stony Brook University

JoVE 57555

 Environment

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

JoVE 57471

 Genetics

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

1Department of Neurobiology, F. M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, 2Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Wellcome Genome Campus, 3Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, 4Functional Proteomics Group, Chester Beatty Laboratories, Institute of Cancer Research

JoVE 57633

 Genetics

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley

JoVE 57350

 Genetics

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto

JoVE 57357

 Immunology and Infection

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School

JoVE 57298

 Immunology and Infection

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School

JoVE 57042

 Immunology and Infection

Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

1Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB), Collège de France, 2Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, 3Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, 4MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, 5Inserm, UMRS1174, Université Paris Sud

JoVE 57728

 Immunology and Infection

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

1Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University Hospital Tübingen, 2Institute of Diabetes Research and Metabolic Diseases (IDM) of the Helmholtz Center Munich, University Tübingen, 3German Center for Diabetes Research (DZD), University Tübingen

JoVE 57642

 Medicine

Treatment of Liver Metastases Using an Internal Target Volume Method for Stereotactic Body Radiotherapy

1Department of Radiation Oncology, Taipei Medical University Hospital, 2Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, 3Taipei Cancer Center, Taipei Medical University, 4Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

JoVE 57050

 Medicine

Use of Two Intracorporeal Ventricular Assist Devices As a Total Artificial Heart

1Division of Cardiothoracic Surgery, Department of Surgery, Duke University, 2Division of Cardiovascular Surgery, Department of Surgery, National Cheng Kung University Hospital and College of Medicine, 3Division of Surgical Sciences, Department of Surgery, Duke University

JoVE 55961

 Medicine

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

1Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, 2DZHK (German Center for Cardiovascular Research), 3Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

JoVE 57617

 Medicine

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

1Department of Radiology, University of California, San Diego, 2Central European Institute of Technology, Brno University of Technology, 3Department of Neurosciences, University of California, San Diego, 4Department of Physics, John Carroll University, 5Department of Biomedical Engineering, Boston University, 6Bioengineering Undergraduate Program, University of California, San Diego, 7Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, 8Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School

JoVE 57214

 Neuroscience

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

1Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 3The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital, Yonsei University College of Medicine, 4Columbia University College of Physicians and Surgeons

JoVE 57123

 Neuroscience

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, 2Institute of Molecular Biology, Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture, National Taiwan University

JoVE 57546

 Neuroscience

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

1Department of Anesthesiology, Ohio State University College of Medicine, 2Medical Student Research Program, Ohio State University College of Medicine, 3Department of Anesthesiology and Pain Medicine, Nationwide Children's Hospital, 4Department of Neuroscience, University of Kentucky Medical Center

JoVE 57391

 Neuroscience

Cannula Implantation into the Cisterna Magna of Rodents

1Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Copenhagen, 2Department of Anesthesiology, Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine, Lund University

JoVE 57378

 Neuroscience

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

1Department of Physiology, School of Basic Medical Sciences, Southern Medical University, 2School of Basic Medical Sciences, Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region, Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences, Southern Medical University

JoVE 56678

 Neuroscience

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