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Table of Contents

Table of Contents: Issue 141, November 2018

Jump to:  Editorial (1) Behavior (9) Biochemistry (11) Bioengineering (4) Biology (6) Cancer Research (3) Chemistry (5) Developmental Biology (7) Engineering (6) Environment (7) Genetics (4) Immunology and Infection (21) Medicine (17) Neuroscience (12) Errata (5)

Gaze in Action: Head-mounted Eye Tracking of Children's Dynamic Visual Attention During Naturalistic Behavior

1Department of Psychological and Brain Sciences, Indiana University, 2Department of Otolaryngology-Head and Neck Surgery, The Ohio State University, 3Department of Psychology, University of California, Riverside

JoVE 58496

 Behavior

Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects

1Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, 2Center for Advanced Research in Education – CIAE, Universidad de Chile, 3Institute of Psychology, Friedrich Schiller University Jena, 4Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, 5Department of Education, Concordia University, 6Berlin School of Mind and Brain, 7Einstein Center for Neurosciences Berlin

JoVE 57694

 Behavior

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 2Institute of Biochemistry, University of Münster, 3Cluster of Excellence 'Cells in Motion', University of Münster

JoVE 58224

 Biochemistry

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

JoVE 58538

 Biochemistry

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

JoVE 57646

 Biochemistry

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University

JoVE 58568

 Biochemistry

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences

JoVE 58327

 Biology

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

JoVE 58564

 Biology

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

1Translational Medicine Program, The Hospital for Sick Children, 2Department of Laboratory Medicine and Pathobiology, University of Toronto, 3Neurosciences & Mental Health Program, The Hospital for Sick Children, 4Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine, Yeungnam University, 5Banting and Best Diabetes Centre, University of Toronto

JoVE 58683

 Biology

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /(T1)Magnetic Resonance Imaging

1Nanobiotechnology, Molecular Imaging and Metabolomics Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2CIC biomaGUNE and CIBER de Enfermedades Respiratorias (CIBERES). Ikerbasque, Basque Foundation for Science, Universidad Complutense de Madrid (UCM), 3Advanced Imaging Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), CIBER de Enfermedades Respiratorias (CIBERES)

JoVE 58269

 Biology

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 2Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 3Department of Molecular Medicine and Surgery, Karolinska Institutet, 4Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, 5Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, 6Hematology Department, Nikea General Hospital, 7Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, 8Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele

JoVE 57787

 Cancer Research

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

1Centre for Biospectroscopy, Monash University, 2Department of Microbiology, Faculty of Medicine, Nursing and Health Sciences, Monash University, 3Centre for Biomedical Research, Burnet Institute, 4Department of Medicine, University of Melbourne

JoVE 56797

 Chemistry

Photogeneration of N-Heterocyclic Carbenes: Application in Photoinduced Ring-Opening Metathesis Polymerization

1ICGM, Université de Montpellier, CNRS, ENSCM, 2Institut de Science des Matériaux de Mulhouse (IS2M UMR 7361 CNRS), Université de Haute-Alsace, 3Université de Strasbourg, 4Laboratoire de Chimie des Polymères Organiques (LCPO UMR 5629 ENSCBP), Université de Bordeaux

JoVE 58539

 Chemistry

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

1Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, 2Department of Dermatology and Venereology, University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

JoVE 58321

 Developmental Biology

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

JoVE 58687

 Developmental Biology

Design and Fabrication of an Optical Fiber Made of Water

1Department of Nanoscience and Nanotechnology, Russell Berrie Nanotechnology Institute (RBNI), Technion - Israel Institute of Technology, 2Department of Material Sciences and Engineering, MIT, 3Centro de Tecnologia Nanofotónica, Universitat Politècnica de València, 4Department of Mechanical Engineering, Technion - Israel Institute of Technology

JoVE 58174

 Engineering

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning, Milligan College, 3Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

JoVE 58486

 Engineering

Production and Measurement of Organic Particulate Matter in the Harvard Environmental Chamber

1School of Engineering and Applied Sciences, Harvard University, 2Department of Environmental Science and Engineering, Gillings School of Global Public Health, University of North Carolina, 3Department of Chemistry, Northwestern University, 4Department of Earth and Planetary Sciences, Harvard University

JoVE 55685

 Environment

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, 2State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, 3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, 4Shanghai Biozeron Biotechnology Co., Ltd

JoVE 58242

 Environment

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology, Rostock University Medical Center

JoVE 58089

 Genetics

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)

JoVE 58467

 Genetics

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

1Department of Medicine, Division of Pulmonary and Critical Care, Women's Guild Lung Institute, Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Cedars-Sinai Medical Center, 3Department of Medicine, Smidt Heart Institute, Cedars-Sinai Medical Center

JoVE 58310

 Immunology and Infection

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

1Department of Psychiatry, Columbia University, 2Department of Pathology & Cell Biology, Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, 4EB Sciences

JoVE 58341

 Immunology and Infection

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 7Department of Applied Physics, Stanford University

JoVE 58305

 Immunology and Infection

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

1Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

JoVE 58509

 Immunology and Infection

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

1Research Service, VA Western New York Healthcare System, 2Department of Ophthalmology, (Ross Eye Institute), Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 3Pharmacology/Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 4Physiology/Biophysics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 5Neuroscience Program, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 6The RNA Institute, University at Buffalo- SUNY, 7The SUNY Eye Institute

JoVE 55894

 Medicine

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

1Department of Hematology, University Hospital of Saint-Etienne, 2Department of Immunology, University Hospital of Grenoble-Alpes, 3Department Hematology, University Hospital of Clermont-Ferrand, 4Department of Immunology, University Hospital of Nice, 5Department of Biopathology, Institute Paoli-Calmettes, 6Department of Hematology, University Hospital of Lille

JoVE 57867

 Medicine

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

1Department of Surgery, Mayo Clinic, 2Midwest Fetal Care Center, Children's Hospitals and Clinics of Minnesota, 3Department of Surgery, Johns Hopkins, 4Pediatric Surgical Associates

JoVE 58399

 Medicine

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

1Graduate Institute of Medical Sciences, National Defense Medical Center, 2Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, 3Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, 4Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 5Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, 6Department of Nursing, Yuanpei University, 7Department of Physiology and Biophysics; Graduate Institute of Physiology, National Defense Medical Center, 8Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center

JoVE 57942

 Medicine

Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury

1Division of Neurosurgery, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, 2Division of PET imaging, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, 3Tokyo Nuclear Services Co. Ltd., 4Department of Neurological Surgery, Graduate School of Medicine, Chiba University

JoVE 58641

 Medicine

One-anastomosis Gastric Bypass (OAGB) in Rats

1Institut national de la santé et de la recherche médicale (Inserm) unité mixte de recherche (UMR) 1149, Unité de formation et de recherche (UFR) de Médecine Paris Diderot, Universit é Paris Diderot, Assistance Publique-Hôpitaux de Paris (AP-HP), 2Department of digestive Surgery, AP-HP Hôpital Européen Georges Pompidou, 3Department of General and Visceral Surgery, Bichat-Claude Bernard University Hospital

JoVE 58776

 Medicine

Left Atrial Stenosis Induced Pulmonary Venous Arterialization and Group 2 Pulmonary Hypertension in Rat

1Department of Medicine, Queen's University, 2Department of Biomedical and Molecular Sciences, Queen's University, 3Department of Cell Physiology, The Jikei University School of Medicine, 4Department of Pediatrics, The Jikei University School of Medicine, 5Department of Cardiovascular Surgery, The Jikei University School of Medicine

JoVE 58787

 Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University

JoVE 57849

 Medicine

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

JoVE 58576

 Neuroscience

Isolation and Culture of Embryonic Mouse Neural Stem Cells

1Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, 2Department of Biology, Faculty of Basic Sciences, Islamic Azad University, 3Department of Biology, ACECR Institute of Higher Education

JoVE 58874

 Neuroscience

A Protocol for Transcranial Photobiomodulation Therapy in Mice

1Neurosciences Research Center, Tabriz University of Medical Sciences, 2ProNeuroLIGHT LLC, 3LiteCure LLC, 4Department of Psychiatry, Harvard Medical School, 5Depression Clinical and Research Program, Department of Psychiatry, Massachusetts General Hospital, 6Center for Anxiety and Traumatic Stress Disorders, Department of Psychiatry, Massachusetts General Hospital, 7Research Institute for Applied Physics and Astronomy, University of Tabriz, 8Department of Medical Physics, Tabriz University of Medical Sciences, 9Department of Medical Bioengineering, Tabriz University of Medical Sciences, 10School of Medical Sciences, University of Aberdeen

JoVE 59076

 Neuroscience

Ethanol-Induced Cervical Sympathetic Ganglion Block Applications for Promoting Canine Inferior Alveolar Nerve Regeneration Using an Artificial Nerve

1Department of Dental Anesthesia, Nippon Dental University Hospital at Tokyo, 2Department of Dental Anesthesiology, Nippon Dental University School of Life Dentistry at Tokyo, 3Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University

JoVE 58039

 Neuroscience

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