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Table of Contents

Table of Contents: Issue 159, May 2020

Jump to:  Behavior (8) Biochemistry (9) Bioengineering (19) Biology (22) Cancer Research (8) Chemistry (7) Developmental Biology (12) Engineering (7) Environment (6) Genetics (8) Immunology and Infection (22) Medicine (23) Neuroscience (25)

Implementation of a Real-Time Psychosis Risk Detection and Alerting System Based on Electronic Health Records using CogStack

1Department of Biostatistics and Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, 2Early Psychosis: Interventions and Clinical-detection (EPIC) lab, Department of Psychosis Studies, Institute of Psychiatry, Psychology & Neuroscience, King's College London, 3National Institute for Health Research, Maudsley Biomedical Research Centre, South London and Maudsley National Health Service (NHS) Foundation Trust, 4Institute of Health Informatics, University College London, 5Department of Psychological Medicine, Institute of Psychiatry, Psychology and Neuroscience, King's College London, 6Health Data Research UK London, University College London, 7OASIS service, South London and Maudsley National Health Service (NHS) Foundation Trust, 8Department of Brain and Behavioral Sciences, University of Pavia

JoVE 60794
 Behavior

Quantitative Static and Dynamic Assessment of Balance Control in Stroke Patients

1Department of Rehabilitation, The Fifth Affiliated Hospital of Guangzhou Medical University, 2The Rehabilitation Medicine Lab of Guangzhou Medical University, 3Experiment Education Model Center of Rehabilitation Medicine, Guangzhou Medical University, 4Department of Rehabilitation Therapy, Guangzhou Medical University, 5College of Physical Education and Sports, Beijing Normal University, 6Department of Rehabilitation, Nanfang Hospital, Southern Medical University


JoVE 60884
 Behavior

An Inertial Measurement Unit Based Method to Estimate Hip and Knee Joint Kinematics in Team Sport Athletes on the Field

1Center for Human Movement Sciences, University Medical Center, University of Groningen, 2Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, 3Emerging Materials, Department of Design Engineering, Delft University of Technology, 4Department of Biomechanical Engineering, Delft University of Technology, 5FIFA Medical Center, Royal Netherlands Football Association, 6Royal Dutch Hockey Association


JoVE 60857
 Behavior

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

1Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, 2Medicity Research Laboratories and Institute of Biomedicine, University of Turku, 3Turku Bioscience Centre, University of Turku and Åbo Akademi University, 4Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital


JoVE 61125
 Biochemistry

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

1Department of Chemistry and Biotechnology, Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster, International Rice Research Institute

JoVE 60602
 Biochemistry

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

JoVE 61054
 Biochemistry

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College, Fudan University


JoVE 60920
 Bioengineering

A Spine Robotic-Assisted Navigation System for Pedicle Screw Placement

1Institute of Biomedical Engineering, National Taiwan University, 2Department of Orthopedic Surgery, National Taiwan University Hospital, Hsin-Chu Branch, 3Department of Orthopedic Surgery, National Taiwan University Hospital, Hsin-Chu Biomedical Park Branch, 4Point Robotics MedTech Inc.

JoVE 60924
 Bioengineering

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University


JoVE 60944
 Bioengineering

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 2University of California Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, 3Department of Pharmaceutical Chemistry, University of California San Francisco, 4California Institute for Quantitative Biosciences, University of California San Francisco, 5Chan Zuckerberg Biohub

JoVE 61014
 Bioengineering

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

1Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications


JoVE 61208
 Bioengineering

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

1Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, 2Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, 3Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), 4Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, 5Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University


JoVE 60859
 Biology

Simple Detection of Primary Cilia by Immunofluorescence

1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University


JoVE 61155
 Biology

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

1The Graduate Program for Neuroscience, Boston University, 2Department of Biology, Boston University, 3Neurophotonics Center, Boston University, 4Center for Systems Neuroscience, Boston University, 5Research and Early Development, Biogen, 6Department Pharmacology and Experimental Therapeutics, Boston University


JoVE 61350
 Biology

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

1Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science, University of Miami, 2Department of Biology, University of Miami, 3The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center, Ben Gurion University of the Negev

JoVE 60446
 Biology

In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish

1Departments of Pharmacology and Medicine, Section of Hematology and Medical Oncology, The Cancer Research Center, Boston University School of Medicine, 2Department of Biomedical Engineering, Tufts University, 3Department of Mechanical Engineering, Tufts University, 4Department of Physics, Tufts University


JoVE 61187
 Cancer Research

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

1Research Unit Analytical BioGeoChemistry, Helmholz Zentrum München-German Research Center for Environmental Health, 2Department of Hematology and Medical Oncology, University Medical Center Göttingen (UMG), 3Institute of Pathology, University Medical Center Göttingen (UMG), 4RECIPE Chemicals + Instruments GmbH


JoVE 61055
 Chemistry

A Salt-Templated Synthesis Method for Porous Platinum-based Macrobeams and Macrotubes

1Department of Chemistry and Life Science, United States Military Academy, 2Photonics Research Center, United States Military Academy, 3U.S. Army Combat Capabilities Development Command-Armaments Center, 4United States Army Research Laboratory-Sensors and Electron Devices Directorate, 5Department of Mathematical Sciences, United States Military Academy


JoVE 61395
 Chemistry

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University

JoVE 60498
 Developmental Biology

Cryogenic Liquid Jets for High Repetition Rate Discovery Science

1SLAC National Accelerator Laboratory, 2University of Alberta, 3Friedrich-Alexander-Universität Erlangen-Nürnberg, 4European XFEL, 5Helmholtz-Zentrum Dresden-Rossendorf, 6Technische Universität Dresden, 7Technische Universität Darmstadt


JoVE 61130
 Engineering

Fabrication of Compressed Hosiery and Measurement of its Pressure Characteristic Exerted on the Lower Limbs

1School of Fashion Engineering, Shanghai University of Engineering Science, 2Textile Industrial Key Laboratory of Ergonomics and Functional Clothing, Shanghai University of Engineering Science, 3Sino-British Joint Lab for Smart Sportswear, Shanghai University of Engineering Science, 4College of Textiles, Donghua University


JoVE 60852
 Engineering

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

1Department of Biological Sciences, University of Cincinnati, 2Department of Biology, Miami University, 3Department of Biology, University of North Carolina at Chapel Hill, 4Department of Neuroscience, The Scripps Research Institute


JoVE 61477
 Environment

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands


JoVE 60905
 Genetics

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University


JoVE 61132
 Genetics

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis

1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, 2Department of Chemical and Biological Engineering, University of Sheffield, 3Hyderabad Eye Research Foundation, L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, 5Department of Molecular Biology and Biotechnology, University of Sheffield, 6Department of Materials Science and Engineering, University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield


JoVE 61156
 Immunology and Infection

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis

JoVE 60728
 Immunology and Infection

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

JoVE 61025
 Immunology and Infection

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

1Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine, University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, 4Department of Immunology, Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine, University of Reims Champagne-Ardenne

JoVE 60810
 Immunology and Infection

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center


JoVE 61068
 Immunology and Infection

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University


JoVE 61183
 Immunology and Infection

Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model

1Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation, Pennsylvania State College of Medicine, 2Department of Biochemistry and Molecular Biology, Pennsylvania State College of Medicine, 3Department of Pharmacology, Pennsylvania State College of Medicine

JoVE 60991
 Medicine

Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System

1Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charité-Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, 2Department of Internal Medicine, Helios Clinic, 3Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, 4Department of Quantitative Health Sciences, University of Massachusetts Medical School


JoVE 60953
 Medicine

Quantification of Biventricular Function and Morphology by Cardiac Magnetic Resonance Imaging in Mice with Pulmonary Artery Banding

1Center for Congenital Heart Diseases, Department of Pediatric Cardiology, University Medical Center Groningen, University of Groningen, 2The Central Animal Facility, University Medical Center Groningen, University of Groningen, 3Gronsai (Groningen Small Animal Imaging Facility), University Medical Center Groningen, University of Groningen, 4Department of Cardiology, University Medical Center Groningen, University of Groningen, 5Department of Radiology, University Medical Center Groningen, University of Groningen


JoVE 60837
 Medicine

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

1Department of Obstetrics, Gynecology and Reproductive Sciences, TB Hair Analysis Laboratory, University of California San Francisco, 2Department of Epidemiology and Biostatistics, University of California San Francisco, 3Department of Medicine, Division of HIV, Infectious Diseases, and Global Medicine, University of California San Francisco, 4Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of California San Francisco

JoVE 60861
 Medicine

Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand

1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, 2Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, 3School of Medicine, Chang Gung University, 4Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, 5Healthy Aging Research Center, Chang Gung University


JoVE 61057
 Medicine

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology


JoVE 61160
 Medicine

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 2Department of Neurosurgery, 10th Military Research Hospital and Polyclinic, 3Division of Preventive Medicine and Healthy Policy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun


JoVE 61260
 Medicine

Elimination of Serotonergic Neurons by Stereotaxic Injection of 5,7-Dihydroxytryptamine in the Dorsal Raphe Nuclei of Mice

1Institute for Regenerative Medicine, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, 2Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University School of Medicine


JoVE 60968
 Neuroscience

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

1Department of Neurology with Experimental Neurology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health


JoVE 61085
 Neuroscience

Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration

1Division of Anesthesia and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Anesthesiology, Yale University School of Medicine, 4Recanati School for Community Health Professions, Faculty of Health Sciences, Ben-Gurion University of the Negev, 5Department of microbiology and immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev

JoVE 61198
 Neuroscience

3D Particle Tracking for Noninvasive In Vivo Analysis of Synaptic Microtubule Dynamics in Dendrites and Neuromuscular Junctions of Drosophila

1Blavatnik Institute of Cell Biology and Program in Neuroscience, Harvard Medical School, 2DRVision Technologies LLC


JoVE 61159
 Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern


JoVE 61157
 Neuroscience

In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris


JoVE 61110
 Neuroscience

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 2Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, 3Instituto de Biologia Molecular, Faculdade de Medicina, Universidade de Lisboa

JoVE 60822
 Neuroscience

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

1School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 2Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, 3School of Basic Medical Sciences, Guangzhou University of Chinese Medicine


JoVE 61177
 Neuroscience

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