Table of Contents: Issue 33, November 2009

The 2009 Lindau Nobel Laureate Meeting: Erwin Neher, Physiology or Medicine 1991

Erwin Neher

JoVE 1563

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Nicolas Di Fiori¹, Amit Meller^1,2

¹Physics Department, Boston University, ²Department of Biomedical Engineering, Boston University

JoVE 1542

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Philippe Desjardins¹, Joel B. Hansen¹, Michael Allen¹

¹Thermo Scientific NanoDrop Products

JoVE 1610

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Andrea Balbo¹, Huaying Zhao¹, Patrick H. Brown¹, Peter Schuck¹

¹National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science

JoVE 1530

Digital Microfluidics for Automated Proteomic Processing

Mais J. Jebrail¹, Vivienne N. Luk^1,2, Steve C. C. Shih^2,3, Ryan Fobel^2,3, Alphonsus H. C. Ng^2,3, Hao Yang¹, Sergio L. S. Freire¹, Aaron R. Wheeler^1,2,3

¹Department of Chemistry, University of Toronto, ²Donnelly Centre for Cellular and Biomolecular Research, ³Institute for Biomaterials and Biomedical Engineering, University of Toronto

JoVE 1603

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Ann S. Cooper¹, Robin L. Cooper¹

¹Department of Biology, University of Kentucky

JoVE 1595

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Sangwon Lee¹, Steven J. Hallam¹

¹Department of Microbiology and Immunology, University of British Columbia - UBC

JoVE 1569

The use of Biofeedback in Clinical Virtual Reality: The INTREPID Project

Claudia Repetto¹, Alessandra Gorini¹, Cinzia Vigna¹, Davide Algeri¹, Federica Pallavicini¹, Giuseppe Riva^1,2

¹Applied Technology for Neuro-Psychology Lab, Istituto Auxologico Italiano, ²Psychology Department, Università Cattolica del Sacro Cuore

JoVE 1554

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Brad Hamilton¹, Qiang Feng¹, Mike Ye¹, G Grant Welstead²

¹Stemgent, ²Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

JoVE 1447

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Ann S. Cooper¹, Kylah E. Rymond¹, Matthew A. Ward¹, Easter L. Bocook¹, Robin L. Cooper¹

¹Department of Biology, University of Kentucky, Lexington

JoVE 1596

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Hideo Hagihara^1,2, Keiko Toyama^1,2, Nobuyuki Yamasaki^1,3, Tsuyoshi Miyakawa^1,2,4,5

¹Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ²Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ³Department of Psychiatry, Graduate School of Medicine, Kyoto University, ⁴Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, ⁵Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

JoVE 1543

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

Wen Xiong¹, Yan Gao¹, Xun Cheng¹, Charles Martin¹, Dongmei Wu¹, Shuyuan Yao¹, Min-Ju Kim², Yang Liu¹

¹Research and Development, Stemgent, ²Product Marketing, Stemgent

JoVE 1550

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Gorazd Pucihar¹, Tadej Kotnik¹, Damijan Miklavčič¹

¹Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana

JoVE 1659

Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord

John D. Houle¹, Arthi Amin¹, Marie-Pascale Cote¹, Michel Lemay¹, Kassi Miller¹, Harra Sandrow¹, Lauren Santi¹, Jed Shumsky¹, Veronica Tom¹

¹Department of Neurobiology and Anatomy, Drexel University College of Medicine

JoVE 1324

Methods for the Study of the Zebrafish Maxillary Barbel

Elizabeth E. LeClair¹, Jacek Topczewski²

¹Department of Biological Sciences, DePaul University, ²Children’s Memorial Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine

JoVE 1558

Aortic Ring Assay

Keren Bellacen¹, Eli C. Lewis¹

¹Department Clinical Biochemistry, Ben-Gurion University

JoVE 1564

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Melissa Paulite¹, Zahra Fakhraai², Boris B. Akhremitchev³, Kerstin Mueller¹, Gilbert C. Walker¹

¹Department of Chemistry, University of Toronto, ²Department of Chemistry, University of Wisconsin, ³Department of Chemistry, Duke University

JoVE 1581

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Daniel S. Dohle¹, Susanne D. Pasa¹, Sebastian Gustmann², Markus Laub³, Josef H. Wissler⁴, Herbert P. Jennissen¹, Nicole Dünker²

¹Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, ²Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, ³Morphoplant GmbH, ⁴ARCONS Institute for Applied Research and Didactics

JoVE 1620

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Sarah E. Staggs¹, Mary Jean See², J P. Dubey³, Eric N. Villegas^2,4

¹Dynamac, Inc., ²Department of Biological Sciences, University of Cincinnati, McMicken College of Arts and Science, ³Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, ⁴National Exposure Research Laboratory, US Environmental Protection Agency

JoVE 1420