Table of Contents: Issue 8, October 2007

JoVE 8th Issue

JoVE 324

Date Published: October 1, 2007  |  doi:10.3791/324  |  Section: JoVE Editorial

Keywords:

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

JoVE 296

Nianzhen Li, Chris Sip, Albert Folch

Dept. of Bioengineering, University of Washington

Date Published: October 1, 2007  |  doi:10.3791/296  |  Section: JoVE General

Keywords: Cellular Biology, BioMEMs, Microvalves, Soft lithography, PDMS, Parallel Mixer, Integrated microfluidic system, Herringbone mixer, Diffusion Gradients, Bioengineering

BioMEMS and Cellular Biology: Perspectives and Applications

JoVE 300

Albert Folch

Department of Biomedical Engineering, University of Washington

Date Published: October 1, 2007  |  doi:10.3791/300  |  Section: JoVE General

Keywords: Biomedical Engineering, BioMEMS, Soft Lithography, Microfluidics, Agrin, Axon Guidance, Olfaction, Interview

Applying Microfluidics to Electrophysiology

JoVE 301

David T. Eddington

Dept. of Bioengineering, University of Illinois, Chicago

Date Published: October 1, 2007  |  doi:10.3791/301  |  Section: JoVE General

Keywords: Neuroscience, Biomedical Engineering, Microfluidics, Slice Recording, Electrophysiology, Neurotransmitter, Bioengineering

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

JoVE 302

Javeed Shaikh Mohammed¹, Hugo Caicedo¹, Christopher P. Fall², David T. Eddington¹

¹Dept. of Bioengineering, University of Illinois, Chicago, ²Department of Anatomy and Cell Biology, University of Illinois, Chicago

Date Published: October 1, 2007  |  doi:10.3791/302  |  Section: JoVE General

Keywords: Neuroscience, Biomedical Engineering, Microfluidics, Slice Recording, Soft Lithography, Electrophysiology, Neurotransmitter, Bioengineering

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

JoVE 305

Joseph Harris¹, Hyuna Lee¹, Christina Tu Tu², David Cribbs³, Carl Cotman³, Noo Li Jeon¹

¹Department of Biomedical Engineering, University of California, Irvine (UCI), ²Stem Cell Research Center, University of California, Irvine (UCI), ³Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/305  |  Section: JoVE General

Keywords: Neuroscience, Biomedical Engineering, Neurons, Axons, Axonal Regeneration, Neuronal Culture, Cell Culture

Windowing Chicken Eggs for Developmental Studies

JoVE 306

Matthew J. Korn, Karina S. Cramer

Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/306  |  Section: JoVE General

Keywords: Developmental Biology, Neuroscience, Chicken, Embryos, Electroporation, In ovo

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

JoVE 307

Matthew J. Korn, Karina S. Cramer

Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/307  |  Section: JoVE General

Keywords: Developmental Biology, Neuroscience, Growth Factor, Heparin-Coated Beads, Chicken, Embryos

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

JoVE 308

Victor Chi, K. George Chandy

Department of Physiology and Biophysics, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/308  |  Section: JoVE General

Keywords: Basic Protocols, Staining, Antibody, Immunohistochemistry

Laser Capture Microdissection of Mammalian Tissue

JoVE 309

Robert A Edwards

Department of Pathology, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/309  |  Section: JoVE General

Keywords: Basic Protocols, Laser Capture Microdissection, Microdissection Techniques, Leica

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

JoVE 325

Christine Beeton, K. George Chandy

Department of Physiology and Biophysics, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/325  |  Section: JoVE General

Keywords: Immunology, Rodent, Hypersensitivity, Mouse, Skin, Immune Reaction, Blood Draw, Serum, Video Protocol, Vaccination, Adjuvant

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

JoVE 326

Christine Beeton, K. George Chandy

Department of Physiology and Biophysics, University of California, Irvine (UCI)

Date Published: October 1, 2007  |  doi:10.3791/326  |  Section: JoVE General

Keywords: Immunology, issue 8, blood, cell isolation, natural killer, lymphocyte, primary cells, negative selection, PBMC, Ficoll gradient, cell separation

Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

JoVE 311

Ravi A. Desai¹, Michael T. Yang¹, Nathan J. Sniadecki², Wesley R. Legant¹, Christopher S. Chen¹

¹Department of Bioengineering, University of Pennsylvania, ²University of Washington

Date Published: October 1, 2007  |  doi:10.3791/311  |  Section: JoVE General

Keywords: Cellular biology, mechanotransduction, traction force, microfabrication

Intravitreous Injection for Establishing Ocular Diseases Model

JoVE 313

Kin Chiu, Raymond Chuen-Chung Chang, Kwok-Fai So

The University of Hong Kong - HKU

Date Published: October 1, 2007  |  doi:10.3791/313  |  Section: JoVE General

Keywords: Neuroscience, eye, injection, rat

Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools

JoVE 314

Utkan Demirci

Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women's Hospital

Date Published: October 1, 2007  |  doi:10.3791/314  |  Section: JoVE General

Keywords: Cellular Biology, microfluidics, diagnostics, capture, blood, HIV, bioengineering

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

JoVE 315

Sang Jun Moon¹, Richard Lin², Utkan Demirci¹

¹Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women's Hospital, ²Massachusetts Institute of Technology

Date Published: October 1, 2007  |  doi:10.3791/315  |  Section: JoVE General

Keywords: Cellular Biology, microfluidic, blood, diagnostics, bioengineering, HIV, Translational Research

Title Cell Encapsulation by Droplets

JoVE 316

Sangjun Moon^1,2, Pei-Ann Lin^1,2, Hasan Onur Keles^1,2, Seung-Schick Yoo³, Utkan Demirci^1,2,4

¹Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women's, Harvard Medical School, ²Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women's Hospital, ³Brigham and Women's Hospital, Harvard Medical School, ⁴Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

Date Published: October 1, 2007  |  doi:10.3791/316  |  Section: JoVE General

Keywords: Cellular Biology, tissue engineering, microfluidics, ejection, imaging, bioengineering

Micro-scale Engineering for Cell Biology

JoVE 317

Joel Voldman

Department of Electrical Engineering and Computer Science, MIT - Massachusetts Institute of Technology

Date Published: October 1, 2007  |  doi:10.3791/317  |  Section: JoVE General

Keywords: Cellular Biology, stem cells, tissue engineering, bioengineering

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

JoVE 318

Nikhil Mittal^1,2, Stephanie Flavin², Joel Voldman²

¹Dept of Physics, MIT - Massachusetts Institute of Technology, ²Department of Electrical Engineering and Computer Science, MIT - Massachusetts Institute of Technology

Date Published: October 1, 2007  |  doi:10.3791/318  |  Section: JoVE General

Keywords: Cellular Biology, tissue engineering, stem cells, patterning, bioengineering, signaling, diffusible, autocrine, juxtacrine

PDMS Device Fabrication and Surface Modification

JoVE 319

Kenneth Kotz, Xuanhong Cheng, Mehmet Toner

Havard Medical School, MGH - Massachusetts General Hospital

Date Published: October 1, 2007  |  doi:10.3791/319  |  Section: JoVE General

Keywords: Cellular Biology, microfluidics, diagnostics, Bioengineering

Cell Capture Using a Microfluidic Device

JoVE 320

Kenneth Kotz, Xuanhong Cheng, Mehmet Toner

Havard Medical School, MGH - Massachusetts General Hospital

Date Published: October 1, 2007  |  doi:10.3791/320  |  Section: JoVE General

Keywords: Cellular Biology, microfluidics, cell isolation, diagnostics, Bioengineering

Development of New Therapeutic Applications Using Microfluidics

JoVE 323

Mehmet Toner

Havard Medical School, MGH - Massachusetts General Hospital

Date Published: October 1, 2007  |  doi:10.3791/323  |  Section: JoVE General

Keywords: Cellular Biology, Microfluidics, Translational Research, Diagnostics, Bioengineering