The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 66, August 2012

Jump to:  JoVE Editorial (1) JoVE General (13) JoVE Neuroscience (9) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (13) JoVE Bioengineering (4) JoVE Applied Physics (5)
 JoVE General

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation


JoVE 4015

1Department of Neurobiology and Anatomy, University of Utah School of Medicine, 2Department of Molecular, Cellular and Developmental Biology, UCSB, 3Howard Hughes Medical Institute, 4Stowers Institute for Medical Research

Date Published: August 6, 2012  |  doi:10.3791/4015  |  Section: JoVE General

 JoVE General

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons


JoVE 4168

1Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, 2Hillman Cancer Center, University of Pittsburgh Cancer Institute, 3Department of Experimental Therapy, The Netherlands Cancer Institute, 4Department of Human Genetics, University of Pittsburgh School of Public Health

Date Published: August 6, 2012  |  doi:10.3791/4168  |  Section: JoVE General

 JoVE General

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes


JoVE 4091

1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

Date Published: August 13, 2012  |  doi:10.3791/4091  |  Section: JoVE General

 JoVE General

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps


JoVE 2810

1The Davis Heart and Lung Research Institute, The Ohio State University, 2Department of Pharmacology, College of Medicine, The Ohio State University

Date Published: August 18, 2012  |  doi:10.3791/2810  |  Section: JoVE General

 JoVE General

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors


JoVE 4081

Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen, Georg August University Medical Center, Göttingen, Germany

Date Published: August 20, 2012  |  doi:10.3791/4081  |  Section: JoVE General

 JoVE General

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts


JoVE 4119

Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University

Date Published: August 27, 2012  |  doi:10.3791/4119  |  Section: JoVE General

 JoVE General

Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species


JoVE 4132

1Department of Neuropathology, Medical School Düsseldorf, Germany, 2Center of Behavioral Neuroscience, University of Düsseldorf

Date Published: August 30, 2012  |  doi:10.3791/4132  |  Section: JoVE General

 JoVE General

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images


JoVE 4233

1Medications Development, Ernest Gallo Clinic and Research Center, University of California, San Francisco, 2Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco, 3Translational Research Institute and the Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

Date Published: August 31, 2012  |  doi:10.3791/4233  |  Section: JoVE General

 JoVE Neuroscience

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development


JoVE 4017

1Northwestern University Feinberg School of Medicine, Children's Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology, Northwestern University Feinberg School of Medicine

Date Published: August 3, 2012  |  doi:10.3791/4017  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area


JoVE 4171

Department of Pharmacology, Toxicology, & Neuroscience, Louisiana State University Health Sciences Center

Date Published: August 10, 2012  |  doi:10.3791/4171  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons


JoVE 4141

1Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

Date Published: August 17, 2012  |  doi:10.3791/4141  |  Section: JoVE Neuroscience

 JoVE Neuroscience

An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice


JoVE 4188

Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine

Date Published: August 17, 2012  |  doi:10.3791/4188  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear


JoVE 3893

1Departments of Psychiatry and Neuroscience, and Friedman Brain Institute, Mt. Sinai School of Medicine, 2Department of Psychology, New York University, 3Department of Psychology and Center for Neural Science, New York University

Date Published: August 24, 2012  |  doi:10.3791/3893  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model


JoVE 4022

Klinik und Poliklinik für Anästhesiologie, University of Wurzburg

Date Published: August 29, 2012  |  doi:10.3791/4022  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines


JoVE 3960

U.S. Army Medical Research Institute of Infectious Diseases

Date Published: August 1, 2012  |  doi:10.3791/3960  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Depletion and Reconstitution of Macrophages in Mice


JoVE 4105

1Department of Graduate Studies, University of British Columbia, 2Department of Molecular Biology, Vrije Universiteit Amsterdam, 3Department of Pediatrics, University of British Columbia

Date Published: August 1, 2012  |  doi:10.3791/4105  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System


JoVE 3683

Department of Immunology, College of Medicine, Mayo Clinic

Date Published: August 8, 2012  |  doi:10.3791/3683  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions


JoVE 4032

Department of Physiology and Pharmacology, University of Calgary

Date Published: August 8, 2012  |  doi:10.3791/4032  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue


JoVE 3814

1Neuroscience Program, Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics, Uniformed Services University, 3Molecular and Cell Biology, Uniformed Services University

Date Published: August 15, 2012  |  doi:10.3791/3814  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells


JoVE 4166

Infectious Disease Research Center, CHUL (CHUQ), Quebec City, Quebec, Canada

Date Published: August 15, 2012  |  doi:10.3791/4166  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry


JoVE 4246

Department of Chemistry, Stony Brook University

Date Published: August 19, 2012  |  doi:10.3791/4246  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children


JoVE 3966

1Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, 2University of Basel, Basel, Switzerland

Date Published: August 22, 2012  |  doi:10.3791/3966  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Plaque Assay for Murine Norovirus


JoVE 4297

Department of Microbiology and Immunology, University of Michigan, Ann Arbor

Date Published: August 22, 2012  |  doi:10.3791/4297  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model


JoVE 3697

Department of Surgery, The Ohio State University Medical Center

Date Published: August 29, 2012  |  doi:10.3791/3697  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model


JoVE 3996

1Department of Applied Physics and Physico-Informatics, Keio University, 2Department of Biochemistry, School of Medicine, Keio University, 3Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project, Japan Science and Technology Agency (JST)

Date Published: August 4, 2012  |  doi:10.3791/3996  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle


JoVE 3973

Department of Plastic and Hand Surgery, University of Freiburg Medical Centre

Date Published: August 5, 2012  |  doi:10.3791/3973  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Use of a Hanging-weight System for Liver Ischemia in Mice


JoVE 2550

1UCH Transplant Center, University of Colorado, Denver, 2Department of Anesthesiology, University of Colorado, Denver

Date Published: August 7, 2012  |  doi:10.3791/2550  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry


JoVE 4108

1Sanford-Burnham Medical Research Institute, 2Division of Dermatology, University of California, San Diego, 3VA San Diego Healthcare Center, 4Moores Cancer Center, University of California, San Diego

Date Published: August 7, 2012  |  doi:10.3791/4108  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

A Human Fallopian Tube Model for Investigation of C. trachomatis Infections


JoVE 4036

1Institute of Medical Microbiology and Hygiene, University of Lübeck, 2Institute of Anatomie, University of Lübeck, 3Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, University of Lübeck, 4Medical Clinic III, University Hospital of Schleswig-Holstein, University of Lübeck

Date Published: August 11, 2012  |  doi:10.3791/4036  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Generation of Alginate Microspheres for Biomedical Applications


JoVE 3388

1Department of Chemical and Biological Engineering, Illinois Institute of Technology, 2Department of Biomedical Engineering, Illinois Institute of Technology, 3Department of Biomedical Engineering, University of California at Irvine, 4Wake Forest Institute for Regenerative Medicine and Department of Biomedical Engineering, Wake Forest University Health Sciences, 5Research Service, Hines Veterans Administration Hospital

Date Published: August 12, 2012  |  doi:10.3791/3388  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound


JoVE 4178

1Department of Medical Physics in Radiology, German Cancer Research Center, Heidelberg, Germany, 2Unit of Chemotherapy and Toxicology, German Cancer Research Center, Heidelberg, Germany

Date Published: August 14, 2012  |  doi:10.3791/4178  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy


JoVE 4218

1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

Date Published: August 14, 2012  |  doi:10.3791/4218  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation


JoVE 3869

1Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven, Belgium, 2Laboratory for Ion Channel Research, Department of Cellular and Molecular Medicine, KU Leuven, Belgium, 3TRP Research Platform Leuven (TRPLe), KU Leuven, Belgium

Date Published: August 21, 2012  |  doi:10.3791/3869  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells


JoVE 4162

Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich

Date Published: August 21, 2012  |  doi:10.3791/4162  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Protocol for Long Duration Whole Body Hyperthermia in Mice


JoVE 3801

1Product Development Cell, National Institute of Immunology, 2Small Animal Facility, National Institute of Immunology

Date Published: August 25, 2012  |  doi:10.3791/3801  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain


JoVE 4204

1Department of Surgery, University of Texas Medical Branch, 2Department of Neuroscience and Cell Biology, University of Texas Medical Branch

Date Published: August 28, 2012  |  doi:10.3791/4204  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression


JoVE 4206

1Department of Preventive Medicine, University of Southern California, 2Institute for Women's Health, University College London

Date Published: August 28, 2012  |  doi:10.3791/4206  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models


JoVE 3981

1Children's Hospital Boston, Harvard Medical School, 2Tufts University

Date Published: August 9, 2012  |  doi:10.3791/3981  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Postproduction Processing of Electrospun Fibres for Tissue Engineering


JoVE 4172

1Materials Science and Engineering, University of Sheffield, 2Department of Biomedical Science, University of Sheffield, 3Department of Chemistry, University of Sheffield

Date Published: August 9, 2012  |  doi:10.3791/4172  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution


JoVE 4161

1Electrical Engineering Department, University of California, Los Angeles, 2Bioengineering Department, University of California, Los Angeles, 3California NanoSystems Institute, University of California, Los Angeles

Date Published: August 16, 2012  |  doi:10.3791/4161  |  Section: JoVE Bioengineering

 JoVE Bioengineering

High-throughput Protein Expression Generator Using a Microfluidic Platform


JoVE 3849

The Mina & Everard Goodman Faculty of Life Sciences, The Nanotechnology Institute, Bar-Ilan University

Date Published: August 23, 2012  |  doi:10.3791/3849  |  Section: JoVE Bioengineering

 JoVE Applied Physics

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water


JoVE 3975

1Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, 2Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, 3Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology

Date Published: August 2, 2012  |  doi:10.3791/3975  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Construction and Testing of Coin Cells of Lithium Ion Batteries


JoVE 4104

1School of Materials Science and Engineering, Clemson University, 2Center for Optical Materials Science and Engineering Technologies, Clemson University

Date Published: August 2, 2012  |  doi:10.3791/4104  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids


JoVE 4296

1Department of Physics, Bowling Green State University, 2The Center for Photochemical Sciences, Bowling Green State University, 3Department of Chemistry, Bowling Green State University

Date Published: August 23, 2012  |  doi:10.3791/4296  |  Section: JoVE Applied Physics

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