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Table of Contents

Table of Contents: Issue 69, November 2012

Jump to:  JoVE Editorial (1) JoVE Biology (12) JoVE Neuroscience (15) JoVE Immunology and Infection (4) JoVE Clinical and Translational Medicine (11) JoVE Bioengineering (5) JoVE Applied Physics (3) JoVE Errata (1)
 JoVE Biology

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

1Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical School, 2Muscle Biology Research Group, University of Missouri-Kansas City, 3Pharmacology division, College of Pharmacy, DHLRI, Ohio State University


JoVE 4198

Date Published: November 1, 2012  |  doi:10.3791/4198  |  Section: JoVE Biology

 JoVE Biology

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

1Department of Biology, University of North Carolina at Charlotte


JoVE 4449

Date Published: November 5, 2012  |  doi:10.3791/4449  |  Section: JoVE Biology

 JoVE Biology

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

1Molecular and Cell Biology, Institute for Biomedical Aging Research, 2Department of Gerontology and Geriatrics, Netherlands Consortium for Healthy Aging, Leiden University Medical Center


JoVE 3327

Date Published: November 10, 2012  |  doi:10.3791/3327  |  Section: JoVE Biology

 JoVE Biology

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

1Department of Molecular Genetics, University of Toronto, 2Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 3Department of Biochemistry, Research and Innovation Centre, University of Regina


JoVE 4056

Date Published: November 12, 2012  |  doi:10.3791/4056  |  Section: JoVE Biology

 JoVE Biology

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 2Deparment of Biochemistry, Research and Innovation Centre, University of Regina, 3Department of Medical Genetics and Microbiology, University of Toronto


JoVE 4057

Date Published: November 12, 2012  |  doi:10.3791/4057  |  Section: JoVE Biology

 JoVE Biology

A Simple Protocol for Extracting Hemocytes from Wild Caterpillars

1Department of Biological Sciences, The George Washington University


JoVE 4173

Date Published: November 15, 2012  |  doi:10.3791/4173  |  Section: JoVE Biology

 JoVE Biology

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

1School of Earth Sciences, The Ohio State University, 2School of Environment & Natural Resources, The Ohio State University, 3Institute of Geology and Geophysics, Chinese Academy of Sciences


JoVE 50123

Date Published: November 15, 2012  |  doi:10.3791/50123  |  Section: JoVE Biology

 JoVE Biology

Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction

1Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, 2Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, 3McCusker Alzheimer's Research foundation, 4School of Medicine and Pharmacology, University of Western Australia, 5Department of Molecular and Biomedical Sciences, University of Adelaide, 6School of Biomedical Sciences, Curtin University of Technology, 7School of Psychiatry and Clinical Neurosciences, University of Western Australia


JoVE 4196

Date Published: November 18, 2012  |  doi:10.3791/4196  |  Section: JoVE Biology

 JoVE Biology

Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT

1Department of Translational Imaging, The Methodist Hospital Research Institute, Houston, 2Diabetes Research Center, The Methodist Hospital Research Institute, Houston


JoVE 4060

Date Published: November 23, 2012  |  doi:10.3791/4060  |  Section: JoVE Biology

 JoVE Biology

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

1The Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University


JoVE 4356

Date Published: November 23, 2012  |  doi:10.3791/4356  |  Section: JoVE Biology

 JoVE Biology

Generation of Mice Derived from Induced Pluripotent Stem Cells

1Dorris Neuroscience Center & Department of Cell Biology, The Scripps Research Institute, 2Mouse Genetics Core Facility, The Scripps Research Institute


JoVE 4003

Date Published: November 29, 2012  |  doi:10.3791/4003  |  Section: JoVE Biology

 JoVE Neuroscience

Creating Objects and Object Categories for Studying Perception and Perceptual Learning

1Brain and Behavior Discovery Institute, Georgia Health Sciences University, 2Vision Discovery Institute, Georgia Health Sciences University, 3Department of Opthalmology, Georgia Health Sciences University, 4Intelligent Systems Laboratory, Palo Alto Research Center, 5Pattern Recognition Systems, Palo Alto Research Center, 6Department of Psychology, University of Minnesota


JoVE 3358

Date Published: November 2, 2012  |  doi:10.3791/3358  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine


JoVE 3631

Date Published: November 3, 2012  |  doi:10.3791/3631  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

1Center for the Neural Basis of Cognition, 2Department of Psychology, University of Pittsburgh, 3Department of Psychology, Carnegie Mellon University, 4Department of Bioengineering, University of Pittsburgh


JoVE 4125

Date Published: November 8, 2012  |  doi:10.3791/4125  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

1Center for Brain Science, Harvard University, 2Program in Neuroscience, Harvard University, 3Department of Organismic and Evolutionary Biology, Harvard University


JoVE 4314

Date Published: November 8, 2012  |  doi:10.3791/4314  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain

1Division of Neurotoxicology, National Center for Toxicological Research, 2Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, 3Office of Planning, Finance, and Information Technology, National Center for Toxicological Research


JoVE 4285

Date Published: November 14, 2012  |  doi:10.3791/4285  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

1Department of Biology, New York University


JoVE 4347

Date Published: November 14, 2012  |  doi:10.3791/4347  |  Section: JoVE Neuroscience

 JoVE Neuroscience

C. elegans Tracking and Behavioral Measurement

1Donnelly Centre, University of Toronto, 2Department of Physics and Astronomy, Vrije Universiteit, 3Okinawa Institute of Science and Technology, 4Department of Physics, University of Toronto


JoVE 4094

Date Published: November 17, 2012  |  doi:10.3791/4094  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

1Division of Neurology, Children's Hospital of Philadelphia, 2Neuroscience Graduate Group, Perelman School of Medicine at the University of Pennsylvania, 3Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania


JoVE 4411

Date Published: November 19, 2012  |  doi:10.3791/4411  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

1Division of Biomedical Sciences, University of California, Riverside, 2Department of Bioengineering, University of California, Riverside


JoVE 50053

Date Published: November 19, 2012  |  doi:10.3791/50053  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

1The Jackson Laboratory


JoVE 4450

Date Published: November 20, 2012  |  doi:10.3791/4450  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

1Department of Neuroscience, University of Florida, 2Department of Psychiatry, University of Florida


JoVE 3798

Date Published: November 21, 2012  |  doi:10.3791/3798  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Analyzing Murine Schwann Cell Development Along Growing Axons

1Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Department of Neuroanatomy, University of Heidelberg, 3FRIAS, University of Freiburg


JoVE 50016

Date Published: November 21, 2012  |  doi:10.3791/50016  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

1Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, 2Paris Diderot University


JoVE 4418

Date Published: November 26, 2012  |  doi:10.3791/4418  |  Section: JoVE Neuroscience

 JoVE Neuroscience

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

1Department of Biology, Emory University, 2Neuroscience Graduate Program, Emory University, 3Program in Neuroscience and Behavioral Biology, Emory University


JoVE 50027

Date Published: November 26, 2012  |  doi:10.3791/50027  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

1Department of Cellular and Molecular Physiology, Yale University School of Medicine


JoVE 4261

Date Published: November 29, 2012  |  doi:10.3791/4261  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Bioluminescent Bacterial Imaging In Vivo

1Cork Cancer Research Centre, BioSciences Institute, University College Cork


JoVE 4318

Date Published: November 4, 2012  |  doi:10.3791/4318  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells

1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, 2Department of Medicine, Division of Nephrology, Baylor College of Medicine, 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine, 5Department of Pediatrics, Baylor College of Medicine, 6Program in Cell and Molecular Biology, Baylor College of Medicine, 7Department of Molecular Virology and Microbiology, Baylor College of Medicine, 8Michael E. DeBakey VA Medical Center


JoVE 4235

Date Published: November 5, 2012  |  doi:10.3791/4235  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Protein Misfolding Cyclic Amplification of Prions

1Department of Civil Engineering, University of Nebraska at Lincoln, 2Department of Medical Microbiology and Immunology, Creighton University


JoVE 4075

Date Published: November 7, 2012  |  doi:10.3791/4075  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

1Institute for Bioscience and Biotechnology Research, University of Maryland


JoVE 4216

Date Published: November 7, 2012  |  doi:10.3791/4216  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

1Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 2CHUQ Research Centre, Laval University


JoVE 4038

Date Published: November 6, 2012  |  doi:10.3791/4038  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Orthotopic Small Bowel Transplantation in Rats

1Department of Surgery, University of Bonn, Germany, 2Department of Surgery, Kyoto University Hospital


JoVE 4102

Date Published: November 6, 2012  |  doi:10.3791/4102  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Training a Sophisticated Microsurgical Technique: Interposition of External Jugular Vein Graft in the Common Carotid Artery in Rats

1European Vascular Center Aachen-Maastricht, Department of Vascular Surgery, University Hospital RWTH Aachen


JoVE 4124

Date Published: November 11, 2012  |  doi:10.3791/4124  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

1Department of Radiology and Bio-X Program Canary Cancer at Stanford for Cancer Early Detection, Stanford University


JoVE 4341

Date Published: November 13, 2012  |  doi:10.3791/4341  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

1Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, 2Departments of Surgery and Medicine, Weill Cornell Medical College, 3Departments of Surgery and Medicine, New York Presbyterian Hospital


JoVE 50086

Date Published: November 13, 2012  |  doi:10.3791/50086  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

1The Jackson Laboratory


JoVE 4201

Date Published: November 20, 2012  |  doi:10.3791/4201  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran


JoVE 4372

Date Published: November 24, 2012  |  doi:10.3791/4372  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

1Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, 2Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University, 3Omics Laboratory, University of Iowa, 4Department of Ophthalmology and Visual Sciences, University of Iowa


JoVE 4286

Date Published: November 25, 2012  |  doi:10.3791/4286  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

1St. Erik's Eye Hospital, Karolinska Institutet, 2Gullstrand lab, Section for Ophthalmology, Department of Neuroscience, Uppsala University


JoVE 4016

Date Published: November 28, 2012  |  doi:10.3791/4016  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

1Department of Surgery, The University of Alabama at Birmingham, 2Department of Medicine, The University of Alabama at Birmingham


JoVE 4338

Date Published: November 28, 2012  |  doi:10.3791/4338  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

1Department of Neurological Surgery, Vanderbilt University School of Medicine


JoVE 50019

Date Published: November 30, 2012  |  doi:10.3791/50019  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

A Microfluidic Device for Studying Multiple Distinct Strains

1Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University


JoVE 4257

Date Published: November 9, 2012  |  doi:10.3791/4257  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

1UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, Université de Lyon, 2Département Biosciences, INSA de Lyon, Université de Lyon, 3INSERM U758, Ecole Normale Supérieure de Lyon, Université de Lyon, 4Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon, Université de Lyon


JoVE 4176

Date Published: November 16, 2012  |  doi:10.3791/4176  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Viral Nanoparticles for In vivo Tumor Imaging

1Department of Biomedical Engineering, Case Western Reserve University, 2Department of Biomedical Engineering, Radiology, and Materials Science and Engineering, Case Western Reserve University


JoVE 4352

Date Published: November 16, 2012  |  doi:10.3791/4352  |  Section: JoVE Bioengineering

 JoVE Applied Physics

A Method to Fabricate Disconnected Silver Nanostructures in 3D

1School of Engineering and Applied Sciences, Harvard University, 2Department of Physics, Harvard University


JoVE 4399

Date Published: November 27, 2012  |  doi:10.3791/4399  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Micro 3D Printing Using a Digital Projector and its Application in the Study of Soft Materials Mechanics

1Department of Mechanical Engineering, Massachusetts Institute of Technology


JoVE 4457

Date Published: November 27, 2012  |  doi:10.3791/4457  |  Section: JoVE Applied Physics

 JoVE Applied Physics

Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities

1School of Physics & Astronomy, University of St Andrews


JoVE 50216

Date Published: November 30, 2012  |  doi:10.3791/50216  |  Section: JoVE Applied Physics

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