Table of Contents: Issue 69, November 2012

November 2012: This Month in JoVE

JoVE 5044

Wendy Chao¹, Aaron Kolski-Andreaco²

¹Department of Ophthalmology, Massachusetts Eye and Ear, ²JoVE Content Production

Date Published: November 1, 2012  |  doi:10.3791/5044  |  Section: JoVE Editorial

Keywords: This Month in JoVE

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

JoVE 4198

Ki Ho Park¹, Leticia Brotto², Oanh Lehoang¹, Marco Brotto², Jianjie Ma¹, Xiaoli Zhao^1,3

¹Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical School, ²Muscle Biology Research Group, University of Missouri-Kansas City, ³Pharmacology division, College of Pharmacy, DHLRI, Ohio State University

Date Published: November 1, 2012  |  doi:10.3791/4198  |  Section: JoVE General

Keywords: Physiology, extensor digitorum longus, soleus, in vitro contractility, calcium signaling, muscle-tendon complex, mechanic alternans

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

JoVE 4266

Yu Seon Chung, Alba Guarné

Department of Biochemistry and Biomedical Sciences, McMaster University

Date Published: November 1, 2012  |  doi:10.3791/4266  |  Section: JoVE General

Keywords: Structural Biology, SeqA, DNA replication, DNA purification, protein-DNA complexes, protein-DNA cocrystallization, X-ray crystallography

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

JoVE 4449

Jeremy Willis*, Darla DeStephanis*, Yogin Patel, Vrushab Gowda, Shan Yan

Department of Biology, University of North Carolina at Charlotte

Date Published: November 5, 2012  |  doi:10.3791/4449  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Cellular Biology, Developmental Biology, DNA damage checkpoint, Xenopus egg extracts, Xenopus laevis, Chk1 phosphorylation, ATR, AT70, MMS, UV, immunoblotting

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

JoVE 3327

Ruth Greussing¹, Hermann Unterluggauer¹, Rafal Koziel¹, Andrea B. Maier², Pidder Jansen-Dürr¹

¹Molecular and Cell Biology, Institute for Biomedical Aging Research, ²Department of Gerontology and Geriatrics, Netherlands Consortium for Healthy Aging, Leiden University Medical Center

Date Published: November 10, 2012  |  doi:10.3791/3327  |  Section: JoVE General

Keywords: Cellular Biology, Molecular Biology, Medicine, Biomedical Engineering, Virology, proteasome activity, lentiviral particles, GFP-dgn, GFP-dgnFS, GFP, human diploid fibroblasts, flow cytometry, plasmid, vector

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

JoVE 4056

Alla Gagarinova*¹, Mohan Babu*^2,3, Jack Greenblatt^1,2, Andrew Emili^1,2

¹Department of Molecular Genetics, University of Toronto, ²Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, ³Department of Biochemistry, Research and Innovation Centre, University of Regina

Date Published: November 12, 2012  |  doi:10.3791/4056  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Medicine, Biochemistry, Microbiology, Aggravating, alleviating, conjugation, double mutant, Escherichia coli, genetic interaction, Gram-negative bacteria, homologous recombination, network, synthetic lethality or sickness, suppression

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

JoVE 4057

Mohan Babu^1,2, Olga Kagan¹, Hongbo Guo¹, Jack Greenblatt^1,3, Andrew Emili^1,3

¹Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, ²Deparment of Biochemistry, Research and Innovation Centre, University of Regina, ³Department of Medical Genetics and Microbiology, University of Toronto

Date Published: November 12, 2012  |  doi:10.3791/4057  |  Section: JoVE General

Keywords: Genetics, Molecular Biology, Medicine, Biochemistry, Microbiology, affinity purification, Escherichia coli, gram-negative bacteria, cytosolic proteins, SPA-tagging, homologous recombination, mass spectrometry, protein interaction, protein complex

A Simple Protocol for Extracting Hemocytes from Wild Caterpillars

JoVE 4173

Teresa M. Stoepler, Julio C. Castillo, John T. Lill, Ioannis Eleftherianos

Department of Biological Sciences, The George Washington University

Date Published: November 15, 2012  |  doi:10.3791/4173  |  Section: JoVE General

Keywords: Cellular Biology, Anatomy, Immunology, Biology, Zoology, Entomology, Cellular immunity, hemocytes, wild caterpillars, non-model insects, Lepidoptera, Lithacodes fasciola, Euclea delphinii, hemolymph, ecoimmunology

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

JoVE 50123

Zachery Oestreicher¹, Steven K. Lower^1,2, Wei Lin³, Brian H. Lower²

¹School of Earth Sciences, The Ohio State University, ²School of Environment & Natural Resources, The Ohio State University, ³Institute of Geology and Geophysics, Chinese Academy of Sciences

Date Published: November 15, 2012  |  doi:10.3791/50123  |  Section: JoVE General

Keywords: Microbiology, Cellular Biology, Earth Sciences, Environmental Sciences, Geology, Magnetotactic bacteria, MTB, bacteria enrichment, racetrack, bacteria isolation, magnetosome, magnetite, hanging drop, magnetism, magnetospirillum, transmission electron microscopy, TEM, light microscopy, pond water, sediment

Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction

JoVE 4196

Avdesh Avdesh*^1,2, Mengqi Chen*^1,3, Mathew T. Martin-Iverson^1,2,4, Alinda Mondal^1,3, Daniel Ong¹, Stephanie Rainey-Smith^1,3, Kevin Taddei^1,3, Michael Lardelli⁵, David M. Groth⁶, Giuseppe Verdile^1,3, Ralph N. Martins^1,2,3,7

¹Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, ²Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, ³McCusker Alzheimer's Research foundation, ⁴School of Medicine and Pharmacology, University of Western Australia, ⁵Department of Molecular and Biomedical Sciences, University of Adelaide, ⁶School of Biomedical Sciences, Curtin University of Technology, ⁷School of Psychiatry and Clinical Neurosciences, University of Western Australia

Date Published: November 18, 2012  |  doi:10.3791/4196  |  Section: JoVE General

Keywords: Basic Protocols, Biology, Marine Biology, Zebrafish, Danio rerio, maintenance, breeding, feeding, raising, larvae, animal model, aquarium

Functional Imaging of Brown Fat in Mice with ¹⁸F-FDG micro-PET/CT

JoVE 4060

Xukui Wang¹, Laurie J. Minze², Zheng-Zheng Shi¹

¹Department of Translational Imaging, The Methodist Hospital Research Institute, Houston, ²Diabetes Research Center, The Methodist Hospital Research Institute, Houston

Date Published: November 23, 2012  |  doi:10.3791/4060  |  Section: JoVE General

Keywords: Molecular Biology, Neuroscience, Anatomy, Physiology, Medicine, Brown adipose tissue, mice, ¹⁸F-Fluorodeoxyglucose, micro-PET, PET, CT, CT scan, tomography, imaging

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

JoVE 4356

Xiuchun Ge, Ping Xu

The Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University

Date Published: November 23, 2012  |  doi:10.3791/4356  |  Section: JoVE General

Keywords: Genetics, Microbiology, Molecular Biology, Biomedical Engineering, Genomics, Streptococcus sanguinis, Streptococcus, Genome-wide gene deletions, genes, High-throughput, PCR

Generation of Mice Derived from Induced Pluripotent Stem Cells

JoVE 4003

Michael J. Boland¹, Jennifer L. Hazen¹, Kristopher L. Nazor¹, Alberto R. Rodriguez², Greg Martin², Sergey Kupriyanov², Kristin K. Baldwin¹

¹Dorris Neuroscience Center & Department of Cell Biology, The Scripps Research Institute, ²Mouse Genetics Core Facility, The Scripps Research Institute

Date Published: November 29, 2012  |  doi:10.3791/4003  |  Section: JoVE General

Keywords: Stem Cell Biology, Molecular Biology, Developmental Biology, Medicine, Cellular Biology, Induced pluripotent stem cells, iPSC, stem cells, reprogramming, developmental potential, tetraploid embryo complementation, mouse

Creating Objects and Object Categories for Studying Perception and Perceptual Learning

JoVE 3358

Karin Hauffen^1,2,3, Eugene Bart⁴, Mark Brady⁵, Daniel Kersten⁶, Jay Hegdé^1,2,3

¹Brain and Behavior Discovery Institute, Georgia Health Sciences University, ²Vision Discovery Institute, Georgia Health Sciences University, ³Department of Opthalmology, Georgia Health Sciences University, ⁴Intelligent Systems Laboratory, Palo Alto Research Center, ⁵Pattern Recognition Systems, Palo Alto Research Center, ⁶Department of Psychology, University of Minnesota

Date Published: November 2, 2012  |  doi:10.3791/3358  |  Section: JoVE Neuroscience

Keywords: Neuroscience, machine learning, brain, classification, category learning, cross-modal perception, 3-D prototyping, inference

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

JoVE 3631

Oleg Alekseev, Anh H. Tran, Jane Azizkhan-Clifford

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

Date Published: November 3, 2012  |  doi:10.3791/3631  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Virology, herpes, cornea, HSV, ex vivo, explant, corneal epithelium, organotypic, keratitis, eye, vision, ophthalmology

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

JoVE 4125

Jeffrey S. Phillips^1,2, Adam S. Greenberg^1,3, John A. Pyles^1,3, Sudhir K. Pathak^1,4, Marlene Behrmann^1,3, Walter Schneider^1,3, Michael J. Tarr^1,3

¹Center for the Neural Basis of Cognition, ²Department of Psychology, University of Pittsburgh, ³Department of Psychology, Carnegie Mellon University, ⁴Department of Bioengineering, University of Pittsburgh

Date Published: November 8, 2012  |  doi:10.3791/4125  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Molecular Biology, Anatomy, Physiology, tractography, connectivity, neuroanatomy, white matter, magnetic resonance imaging, MRI

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

JoVE 4314

Timothy M. Otchy^1,2, Bence P. Ӧlveczky^1,3

¹Center for Brain Science, Harvard University, ²Program in Neuroscience, Harvard University, ³Department of Organismic and Evolutionary Biology, Harvard University

Date Published: November 8, 2012  |  doi:10.3791/4314  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Medicine, Anatomy, Mechanical Engineering, microdrive, in-vivo chronic recording, electrophysiology, songbirds

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain

JoVE 4285

John F. Bowyer¹, Monzy Thomas¹, Tucker A. Patterson¹, Nysia I. George², Jeffrey A. Runnells³, Mark S. Levi¹

¹Division of Neurotoxicology, National Center for Toxicological Research, ²Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, ³Office of Planning, Finance, and Information Technology, National Center for Toxicological Research

Date Published: November 14, 2012  |  doi:10.3791/4285  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Medicine, Anatomy, Physiology, Toxicology, brain, dissection, choroid plexus, meninges and associated vasculature

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

JoVE 4347

Hui-Yi Hsiao*, Robert J. Johnston Jr.*, David Jukam*, Daniel Vasiliauskas*, Claude Desplan, Jens Rister

Department of Biology, New York University

Date Published: November 14, 2012  |  doi:10.3791/4347  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Anatomy, Physiology, Immunology, Developmental Biology, Drosophila, retina, photoreceptor, imaginal disc, larva, pupa, confocal microscopy, immunohistochemistry

C. elegans Tracking and Behavioral Measurement

JoVE 4094

Jirapat Likitlersuang¹, Greg Stephens^2,3, Konstantine Palanski¹, William S. Ryu^1,4

¹Donnelly Centre, University of Toronto, ²Department of Physics and Astronomy, Vrije Universiteit, ³Okinawa Institute of Science and Technology, ⁴Department of Physics, University of Toronto

Date Published: November 17, 2012  |  doi:10.3791/4094  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physics, Biophysics, Anatomy, Microscopy, Ethology, Behavior, Machine Vision, C. elegans, animal model

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

JoVE 4411

Colin J. Smith^1,2, Brian N. Johnson¹, Jaclynn A. Elkind¹, Jill M. See¹, Guoxiang Xiong¹, Akiva S. Cohen^1,3

¹Division of Neurology, Children's Hospital of Philadelphia, ²Neuroscience Graduate Group, Perelman School of Medicine at the University of Pennsylvania, ³Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania

Date Published: November 19, 2012  |  doi:10.3791/4411  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Medicine, Anatomy, Physiology, hippocampus, traumatic brain injury, electrophysiology, patch clamp, voltage sensitive dye, extracellular recording, high-performance liquid chromatography, gas chromatography-mass spectrometry

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

JoVE 50053

Jenny I. Szu¹, Melissa M. Eberle², Carissa L. Reynolds², Mike S. Hsu¹, Yan Wang², Christian M. Oh², M. Shahidul Islam², B. Hyle Park², Devin K. Binder¹

¹Division of Biomedical Sciences, University of California, Riverside, ²Department of Bioengineering, University of California, Riverside

Date Published: November 19, 2012  |  doi:10.3791/50053  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Bioengineering, Medicine, Biomedical Engineering, Anatomy, Physiology, Thinned-skull cortical window (TSCW), Optical coherence tomography (OCT), Spectral-domain OCT (SD-OCT), cerebral cortex, brain, imaging, mouse model

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

JoVE 4450

Yun Li, Brittany D. Roy, Wei Wang, Lifeng Zhang, Stephen B. Sampson, Da-Ting Lin

The Jackson Laboratory

Date Published: November 20, 2012  |  doi:10.3791/4450  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cellular Biology, Bioengineering, Medicine, primary cultured mouse neuron, superecliptic pHluorin, receptor, plasma membrane insertion, total internal reflection fluorescence microscopy, neurons, mice, pHlourin-tagged, plasma membrane

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

JoVE 3798

Kaustuv Saha¹, Jarod Swant¹, Habibeh Khoshbouei^1,2

¹Department of Neuroscience, University of Florida, ²Department of Psychiatry, University of Florida

Date Published: November 21, 2012  |  doi:10.3791/3798  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Cellular Biology, Physiology, Medicine, Simultaneous Patch Clamp and Voltametry, In Vitro Voltametry, Dopamine, Oxidation, Whole-cell Patch Clamp, Dopamine Transporter, Reverse transport, Efflux

Analyzing Murine Schwann Cell Development Along Growing Axons

JoVE 50016

Stephan Heermann^1,2, Kerstin Krieglstein^1,3

¹Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, ²Department of Neuroanatomy, University of Heidelberg, ³FRIAS, University of Freiburg

Date Published: November 21, 2012  |  doi:10.3791/50016  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Medicine, Cellular Biology, Anatomy, Physiology, Developmental Biology, Schwann cell, migration, time-lapse, SCG, neurons, axons, mouse

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

JoVE 4418

Ulrike Pannasch*¹, Jérémie Sibille*^1,2, Nathalie Rouach¹

¹Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, ²Paris Diderot University

Date Published: November 26, 2012  |  doi:10.3791/4418  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Physiology, Anatomy, Medicine, hippocampus preparation, acute brain slice, electrophysiology, patch-clamp, neurons, astrocytes, astroglial, neuroglial interactions, glutamate transporter current, potassium current, paired recordings, synaptic activity, synaptically-evoked responses

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

JoVE 50027

Lukas A. Hoffmann^1,2, Conor W. Kelly^1,3, David A. Nicholson^1,2, Samuel J. Sober¹

¹Department of Biology, Emory University, ²Neuroscience Graduate Program, Emory University, ³Program in Neuroscience and Behavioral Biology, Emory University

Date Published: November 26, 2012  |  doi:10.3791/50027  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Anatomy, Physiology, Zoology, Behavior, Songbird, psychophysics, auditory feedback, biology, sensorimotor learning

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

JoVE 4261

Marko Popovic, Xin Gao, Dejan Zecevic

Department of Cellular and Molecular Physiology, Yale University School of Medicine

Date Published: November 29, 2012  |  doi:10.3791/4261  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Medicine, Physiology, Molecular Biology, Cellular Biology, voltage-sensitive dyes, brain, imaging, dendritic spines, axons, dendrites, neurons

Bioluminescent Bacterial Imaging In Vivo

JoVE 4318

Chwanrow K. Baban*, Michelle Cronin*, Ali R. Akin, Anne O'Brien, Xuefeng Gao, Sabin Tabirca, Kevin P. Francis, Mark Tangney

Cork Cancer Research Centre, BioSciences Institute, University College Cork

Date Published: November 4, 2012  |  doi:10.3791/4318  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Cancer Biology, Genetics, Gene Therapy, Cancer, Vector, Lux, Optical Imaging, Luciferase

piggyBac Transposon System Modification of Primary Human T Cells

JoVE 4235

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, ²Department of Medicine, Division of Nephrology, Baylor College of Medicine, ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine, ⁵Department of Pediatrics, Baylor College of Medicine, ⁶Program in Cell and Molecular Biology, Baylor College of Medicine, ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine, ⁸Michael E. DeBakey VA Medical Center

Date Published: November 5, 2012  |  doi:10.3791/4235  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Medicine, Genetics, Cellular Biology, Virology, Human T cells, Transposons, piggyBac, transgene

Protein Misfolding Cyclic Amplification of Prions

JoVE 4075

Samuel E. Saunders¹, Jason C. Bartz², Ronald A. Shikiya²

¹Department of Civil Engineering, University of Nebraska at Lincoln, ²Department of Medical Microbiology and Immunology, Creighton University

Date Published: November 7, 2012  |  doi:10.3791/4075  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Genetics, Virology, prion, prion detection, sonication, PrP^C, PrP^Sc, strain, in vitro, PMCA, sPMCA

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

JoVE 4216

Ryan D. Heselpoth, Daniel C. Nelson

Institute for Bioscience and Biotechnology Research, University of Maryland

Date Published: November 7, 2012  |  doi:10.3791/4216  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Molecular Biology, Genetics, Microbiology, directed evolution, thermal behavior, thermostability, endolysin, enzybiotic, bacteriolytic, antimicrobial, therapeutic, PlyC

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

JoVE 4038

Estelle Rousselet¹, Jasna Kriz², Nabil G. Seidah¹

¹Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, ²CHUQ Research Centre, Laval University

Date Published: November 6, 2012  |  doi:10.3791/4038  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Biochemistry, Anatomy, Physiology, transient ischemic stroke, middle cerebral artery occlusion, intraluminal model, neuroscore, cresyl violet staining, mice, imaging

Orthotopic Small Bowel Transplantation in Rats

JoVE 4102

Koji Kitamura*^1,2, Martin W. von Websky*¹, Ichiro Ohsawa¹, Azin Jaffari¹, Thomas C. Pech¹, Tim Vilz¹, Sven Wehner¹, Shinji Uemoto², Joerg C. Kalff¹, Nico Schaefer¹

¹Department of Surgery, University of Bonn, Germany, ²Department of Surgery, Kyoto University Hospital

Date Published: November 6, 2012  |  doi:10.3791/4102  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Immunology, intestinal transplantation, orthotopic small bowel transplantation, acute rejection, small bowel, surgery, operation, rat

Training a Sophisticated Microsurgical Technique: Interposition of External Jugular Vein Graft in the Common Carotid Artery in Rats

JoVE 4124

Karina Schleimer, Jochen Grommes, Andreas Greiner, Houman Jalaie, Johannes Kalder, Stephan Langer, Thomas A. Koeppel, Michael Jacobs, Maria Kokozidou

European Vascular Center Aachen-Maastricht, Department of Vascular Surgery, University Hospital RWTH Aachen

Date Published: November 11, 2012  |  doi:10.3791/4124  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Immunology, Surgery, microsurgery, neointimal hyperplasia, venous interposition graft, external jugular vein, common carotid artery, rat

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

JoVE 4341

Yingding Xu, Hongguang Liu, Edwin Chang, Han Jiang, Zhen Cheng

Department of Radiology and Bio-X Program Canary Cancer at Stanford for Cancer Early Detection, Stanford University

Date Published: November 13, 2012  |  doi:10.3791/4341  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, Molecular Biology, Cerenkov Luminescence Imaging, CLI, cancer therapy monitoring, optical imaging, PET, radionuclides, Avastin, imaging

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

JoVE 50086

Sharanya Iyengar¹, Yariv Houvras^2,3, Craig J. Ceol¹

¹Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, ²Departments of Surgery and Medicine, Weill Cornell Medical College, ³Departments of Surgery and Medicine, New York Presbyterian Hospital

Date Published: November 13, 2012  |  doi:10.3791/50086  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, Genetics, Molecular Biology, Melanoma, zebrafish, Danio rerio, mitfa, melanocytes, tumor model, miniCoopR

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

JoVE 4201

Lifeng Zhang*, Andree Lapierre*, Brittany Roy, Maili Lim, Jennifer Zhu, Wei Wang, Stephen B. Sampson, Kyuson Yun, Bonnie Lyons, Yun Li, Da-Ting Lin

The Jackson Laboratory

Date Published: November 20, 2012  |  doi:10.3791/4201  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Cancer Biology, Stem Cell Biology, Bioengineering, Biomedical Engineering, polished and reinforced thin-skull cranial window, two-photon microscopy, glioma stem cell, vasculature, PoRTS

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

JoVE 4372

Razieh Karamzadeh¹, Mohamadreza Baghaban Eslaminejad¹, Reza Aflatoonian²

¹Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, ²Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Date Published: November 24, 2012  |  doi:10.3791/4372  |  Section: JoVE Clinical and Translational Medicine

Keywords: Stem Cell Biology, Medicine, Developmental Biology, Cellular Biology, Bioengineering, Dental pulp tissue, Human third molar, Human dental pulp stem cells, hDPSC, Odontoblasts, Outgrown stem cells, MSC, differentiation

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

JoVE 4286

Katherine J. Wert^1,2, Jessica M. Skeie^3,4, Richard J. Davis¹, Stephen H. Tsang^1,3, Vinit B. Mahajan^3,4

¹Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, ²Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University, ³Omics Laboratory, University of Iowa, ⁴Department of Ophthalmology and Visual Sciences, University of Iowa

Date Published: November 25, 2012  |  doi:10.3791/4286  |  Section: JoVE Clinical and Translational Medicine

Keywords: Stem Cell Biology, Medicine, Ophthalmology, Anatomy, Physiology, Cellular Biology, Genetics, mouse, subretinal injection, iPS cells, stem cells, retina, eye, gene therapy

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

JoVE 4338

Lingling Guo¹, Anupam Agarwal², James F. George¹

¹Department of Surgery, The University of Alabama at Birmingham, ²Department of Medicine, The University of Alabama at Birmingham

Date Published: November 28, 2012  |  doi:10.3791/4338  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Anatomy, Physiology, Surgery, Vascular surgery, mice, artery, aorta, transplantation, vascular disease, aortic transplantation, orthotopic, mouse vascular disease models

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

JoVE 4016

Konstantin Galichanin^1,2, Nooshin Talebizadeh², Per Söderberg²

¹St. Erik's Eye Hospital, Karolinska Institutet, ²Gullstrand lab, Section for Ophthalmology, Department of Neuroscience, Uppsala University

Date Published: November 28, 2012  |  doi:10.3791/4016  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Molecular Biology, Ophthalmology, Immunology, UVR-B, lens, cataract, qRT-PCR, PCR, immunohistochemistry, rat restrainer, animal model

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

JoVE 50019

Jacob A. Martin, Alexander S. Maris, Moneeb Ehtesham, Robert J. Singer

Department of Neurological Surgery, Vanderbilt University School of Medicine

Date Published: November 30, 2012  |  doi:10.3791/50019  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Neuroscience, Immunology, Cancer Biology, Blood-brain barrier disruption, neurovascular, endovascular, intra-arterial, neurosurgery, oncology, neuro-oncology, animal model, rat

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

JoVE 4398

Michael D. Leipold, Holden T. Maecker

Human Immune Monitoring Center, Institute for Immunity, Transplantation, and Infection, Stanford University

Date Published: November 2, 2012  |  doi:10.3791/4398  |  Section: JoVE Bioengineering

Keywords: Bioengineering, CyTOF, mass cytometry, PBMCs, ICP-MS, multiparametric

A Microfluidic Device for Studying Multiple Distinct Strains

JoVE 4257

Guy Aidelberg*, Yifat Goldshmidt*, Iftach Nachman

Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University

Date Published: November 9, 2012  |  doi:10.3791/4257  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biochemistry, Molecular Biology, Microbiology, Microfluidics, PDMS, Time lapse fluorescent microscopy, S. cerevisiae, imaging, bacteria, strains

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

JoVE 50117

Douglas S. Martin, Lu Yu, Brian L. Van Hoozen

Department of Physics, Lawrence University

Date Published: November 9, 2012  |  doi:10.3791/50117  |  Section: JoVE Bioengineering

Keywords: Biophysics, Bioengineering, Physics, Molecular Biology, Cellular Biology, microtubule, persistence length, flexural rigidity, gliding assay, mechanics, cytoskeleton, actin

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

JoVE 4176

Benoît Drogue¹, Philippe Thomas², Laurent Balvay³, Claire Prigent-Combaret¹, Corinne Dorel⁴

¹UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, Université de Lyon, ²Département Biosciences, INSA de Lyon, Université de Lyon, ³INSERM U758, Ecole Normale Supérieure de Lyon, Université de Lyon, ⁴Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon, Université de Lyon

Date Published: November 16, 2012  |  doi:10.3791/4176  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Microbiology, Molecular Biology, curli, cobalt, biofilm, Escherichia coli, synthetic operon, synthetic biology, adherence assay, biofilm quantification, microscopy

Viral Nanoparticles for In vivo Tumor Imaging

JoVE 4352

Amy M. Wen¹, Karin L. Lee¹, Ibrahim Yildiz¹, Michael A. Bruckman¹, Sourabh Shukla¹, Nicole F. Steinmetz²

¹Department of Biomedical Engineering, Case Western Reserve University, ²Department of Biomedical Engineering, Radiology, and Materials Science and Engineering, Case Western Reserve University

Date Published: November 16, 2012  |  doi:10.3791/4352  |  Section: JoVE Bioengineering

Keywords: Cancer Biology, Bioengineering, Biomedical Engineering, Molecular Biology, Virology, Oncology, Viral nanoparticles, bioconjugate chemistry, tumor xenograft mouse model, fluorescence imaging

A Method to Fabricate Disconnected Silver Nanostructures in 3D

JoVE 4399

Kevin Vora¹, SeungYeon Kang¹, Eric Mazur^1,2

¹School of Engineering and Applied Sciences, Harvard University, ²Department of Physics, Harvard University

Date Published: November 27, 2012  |  doi:10.3791/4399  |  Section: JoVE Applied Physics

Keywords: Physics, Materials Science, Engineering, Nanotechnology, nanofabrication, microfabrication, 3D fabrication, polymer, silver, femtosecond laser processing, direct laser writing, multiphoton lithography, nonlinear absorption

Micro 3D Printing Using a Digital Projector and its Application in the Study of Soft Materials Mechanics

JoVE 4457

Howon Lee, Nicholas X. Fang

Department of Mechanical Engineering, Massachusetts Institute of Technology

Date Published: November 27, 2012  |  doi:10.3791/4457  |  Section: JoVE Applied Physics

Keywords: Mechanical Engineering, Materials Science, Physics, Chemical Engineering, 3D printing, stereo-lithography, photo-polymerization, gel, swelling, elastic instability, buckling, pattern formation

Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities

JoVE 50216

Christopher Paul Reardon, Isabella H. Rey, Karl Welna, Liam O'Faolain, Thomas F. Krauss

School of Physics & Astronomy, University of St Andrews

Date Published: November 30, 2012  |  doi:10.3791/50216  |  Section: JoVE Applied Physics

Keywords: Physics, Optics and Photonics, Astronomy, light scattering, light transmission, optical waveguides, photonics, photonic crystals, Slow-light, Cavities, Waveguides, Silicon, SOI, Fabrication, Characterization

Erratum: Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Date Published: November 13, 2012  |  doi:10.3791/5045

Keywords: Errata