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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 62, April 2012

Jump to:  JoVE Editorial (1) JoVE General (17) JoVE Neuroscience (10) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (10) JoVE Bioengineering (4) JoVE Errata (6)
 JoVE General

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models


JoVE 3868

,

Basic Medical Sciences, University of Arizona College of Medicine - Phoenix

Date Published: April 3, 2012  |  doi:10.3791/3868  |  Section: JoVE General

Keywords: Cellular Biology, Rotating wall vessel bioreactor, female reproductive tract, human epithelial cells, three-dimensional in vitro cell culture, organotypic mucosal models, vaginal epithelial cells, microbicide, herpes simplex virus, toxicology, host-pathogen interactions, hormone receptors

 JoVE General

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)


JoVE 3513

1, 2,3, 1

1Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, 2Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, 3Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud

Date Published: April 6, 2012  |  doi:10.3791/3513  |  Section: JoVE General

Keywords: Cellular Biology, Lipid rafts, plasma membrane, diffusion times, confocal microscopy, fluorescence correlation spectroscopy (FCS)

 JoVE General

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy


JoVE 3163

*1, *2, 1, 3, 2,3,4, 1,2

1Biosphere Oriented Biology Research Unit, RIKEN Advanced Science Institute, 2Graduate School of Nanobioscience, Yokohama City University, 3Advanced NMR Metabomics Research Team, RIKEN Plant Science Center, 4Graduate School of Bioagricultural Science, Nagoya University

Date Published: April 7, 2012  |  doi:10.3791/3163  |  Section: JoVE General

Keywords: Molecular Biology, environmental metabolomics, metabolic profiling, microbial ecology, plankton, NMR spectroscopy, PCA

 JoVE General

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood


JoVE 3872

, ,

Herman B Wells Center for Pediatric Research, Indiana University School of Medicine

Date Published: April 13, 2012  |  doi:10.3791/3872  |  Section: JoVE General

Keywords: Cellular Biology, Endothelial colony-forming cells (ECFCs), endothelial progenitor cells (EPCs), single cell colony forming assay, post-natal vasculogenesis, cell culture, cloning

 JoVE General

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana


JoVE 3790

1, 2, 1, 1, 1

1Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, 2Regional Agency for Environmental Protection in Emilia-Romagna

Date Published: April 14, 2012  |  doi:10.3791/3790  |  Section: JoVE General

Keywords: Chemistry, Artemia franciscana, bioassays, chemical substances, crustaceans, marine environment

 JoVE General

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels


JoVE 3850

, ,

Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

Date Published: April 24, 2012  |  doi:10.3791/3850  |  Section: JoVE General

Keywords: Medicine, G protein-gated inward rectifier K+ (GIRK) channels, clonal cell lines, drug screening, fluorescent dyes, K+ channel modulators, Pharmacology

 JoVE General

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue


JoVE 3764

1, 2, 1

1Howard Hughes Medical Institute, Department of Neurobiology, Duke University, 2Department of Biological Sciences, Hokkaido University

Date Published: April 27, 2012  |  doi:10.3791/3764  |  Section: JoVE General

Keywords: Neuroscience, in situ hybridization, radioactive, riboprobes, vertebrate, embryo, silver emulsion, darkfield, genetics

 JoVE General

The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity


JoVE 3674

, ,

Department of Physiology and Pharmacology, Center for Cardiovascular and Respiratory Sciences, West Virginia University

Date Published: April 28, 2012  |  doi:10.3791/3674  |  Section: JoVE General

Keywords: Physiology, isolated microvessel preparation, skeletal muscle arterioles, resistance arteriole, microcirculation, arteriolar wall mechanics

 JoVE General

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation


JoVE 3770

*1, *1,2,3, 1, 1, 1, 1, 1, 1,3

1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore

Date Published: April 30, 2012  |  doi:10.3791/3770  |  Section: JoVE General

Keywords: Genetics, ChIP, ChIA-PET, Chromatin Interactions, Genomics, Next-Generation Sequencing

 JoVE Neuroscience

Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique


JoVE 3862

*1, *1, 2, 1

1Monell Chemical Senses Center, 2Department of Physiology, Development & Neuroscience, Physiological Laboratory, University of Cambridge

Date Published: April 5, 2012  |  doi:10.3791/3862  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Olfactory receptor neurons, ORN, suction pipette technique, receptor current, action potentials, signal transduction, electrophysiology, chemoreceptors

 JoVE Neuroscience

Wholemount Immunohistochemistry for Revealing Complex Brain Topography


JoVE 4042

1, 1, 2, 1

1Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary

Date Published: April 5, 2012  |  doi:10.3791/4042  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Gene expression, antibodies, compartmentalization, brain topography, circuitry, neuroanatomy

 JoVE Neuroscience

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium


JoVE 3920

1, 1, 2, 1

1Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK, 2Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK

Date Published: April 12, 2012  |  doi:10.3791/3920  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Live imaging, chick, embryo, spinal cord, time-lapse, developing neuroepithelium

 JoVE Neuroscience

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System


JoVE 3597

1, 2, 1, 2, 1

1Department of Biological Sciences, Florida Atlantic University, 2Department of Chemistry & Biochemistry, Florida Atlantic University

Date Published: April 15, 2012  |  doi:10.3791/3597  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Drosophila melanogaster, Giant Fiber Circuit, screening, in vivo, nanoinjection, electrophysiology, modulatory compounds, biochemistry

 JoVE Neuroscience

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes


JoVE 4008

,

Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Date Published: April 19, 2012  |  doi:10.3791/4008  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Retrograde labeling, Fluorescent dyes, Spinal cord, Nerves, Spinal tracts, Optical imaging, Electrophysiology, Calcium-sensitive dyes

 JoVE Neuroscience

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry


JoVE 3712

1,2, 1, 1, 1

1Department of Neurosurgery, The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Date Published: April 22, 2012  |  doi:10.3791/3712  |  Section: JoVE Neuroscience

Keywords: Neuroscience, neural Stem Cell, Neuronal Progenitor Cells, Flow Cytometry, Isolation, Enrichment

 JoVE Neuroscience

Nerve Excitability Assessment in Chemotherapy-induced Neurotoxicity


JoVE 3439

1,2, 3, 1,2

1Prince of Wales Clinical School, University of New South Wales, 2Neuroscience Research Australia, University of New South Wales, 3School of Medical Sciences, University of New South Wales

Date Published: April 26, 2012  |  doi:10.3791/3439  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Chemotherapy, Neurotoxicity, Neuropathy, Nerve excitability, Ion channel function, Oxaliplatin, oncology, medicine

 JoVE Immunology and Infection

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling


JoVE 3819

*1, *1, 2, 1, 1, 1, 1

1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University

Date Published: April 2, 2012  |  doi:10.3791/3819  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Adeno-associated virus, AAV, gene therapy, synthetic biology, viral vector, molecular evolution, DNA shuffling

 JoVE Immunology and Infection

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation


JoVE 3823

1, 1, 2, 3, 2,4,5,6,7, 1, 1

1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

Date Published: April 2, 2012  |  doi:10.3791/3823  |  Section: JoVE Immunology and Infection

Keywords: Immunology, neuron cell culture, Herpes Simplex Virus (HSV), molecular biology, virology

 JoVE Immunology and Infection

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles


JoVE 3171

, , , ,

System Biosciences

Date Published: April 8, 2012  |  doi:10.3791/3171  |  Section: JoVE Immunology and Infection

Keywords: Immunology, lentivector, virus packaging, pseudovirus production, lentiviral packaging, HIV-based lentivector, lentiviral delivery, lentiviral transduction, lentivirus concentration, stable expression, stable cell lines

 JoVE Immunology and Infection

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge


JoVE 3778

1, 2, 2

1Department of Medicine, Section of Cardiology, St. John-Providence Health System, 2Department of Immunology and Microbiology, Wayne State University School of Medicine

Date Published: April 9, 2012  |  doi:10.3791/3778  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Autoimmune diseases, experimental autoimmune encephalomyelitis, immunization, myelin basic protein, adoptive transfer, paralysis

 JoVE Immunology and Infection

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells


JoVE 3738

, , , ,

Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

Date Published: April 16, 2012  |  doi:10.3791/3738  |  Section: JoVE Immunology and Infection

Keywords: Immunology, regulatory T cell, iTreg, immunosuppression, human, suppressor activity

 JoVE Immunology and Infection

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood


JoVE 3741

,

Department of Internal Medicine, Yale University School of Medicine

Date Published: April 16, 2012  |  doi:10.3791/3741  |  Section: JoVE Immunology and Infection

Keywords: Immunology, monocyte, dendritic cells, Toll-like receptors, fluorescent imaging, signaling, FACS, aging

 JoVE Immunology and Infection

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs


JoVE 3533

1, 1,2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 1

1Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, 2School of Agrarian Sciences, University of Antioquia

Date Published: April 21, 2012  |  doi:10.3791/3533  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Cutaneous leishmaniasis, hamster, Leishmania, antileishmanial drugs

 JoVE Immunology and Infection

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems


JoVE 3518

*1,2, *1, 1,2, 1,2, 1,2

1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ)

Date Published: April 23, 2012  |  doi:10.3791/3518  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Recombinant parvovirus, adenovirus, virus production, pXX6, virus helper, virology, oncology

 JoVE Immunology and Infection

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays


JoVE 3727

*, *, , ,

Plant Pathology and Microbiology, Texas A&M University

Date Published: April 23, 2012  |  doi:10.3791/3727  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Mycotoxins, sporogenesis, Aspergillus flavus, Fusarium verticillioides, aflatoxin, fumonisin, plant-microbe interactions, plant biology

 JoVE Clinical and Translational Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats


JoVE 3247

1,2, 1, 1, 1

1Center for Brain and Spinal Cord Repair, School of Allied Medical Professions, The Ohio State University, 2Spinal Cord Research Center, Drexel University College of Medicine

Date Published: April 4, 2012  |  doi:10.3791/3247  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Rat, neuropathic pain, allodynia, tactile sensation, spinal cord injury, SCI, von Frey monofilaments

 JoVE Clinical and Translational Medicine

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography


JoVE 3680

1, 2, 1, 2, 1, 3, 1, 4, 2

1Carestream Molecular Imaging, 2Department of Chemistry and Biochemistry, University of Notre Dame, 3Freimann Life Science Center, University of Notre Dame, 4Research and Development, Oncovision, GEM-Imaging S.A.

Date Published: April 4, 2012  |  doi:10.3791/3680  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, X-ray computed tomography (CT), image analysis, in vivo, obesity, metabolic disorders

 JoVE Clinical and Translational Medicine

Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects


JoVE 3552

, , , ,

Department of Orthopaedic Surgery, University of California, San Francisco

Date Published: April 11, 2012  |  doi:10.3791/3552  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Bone fracture, intramembranous ossification, distraction osteogenesis, bone healing

 JoVE Clinical and Translational Medicine

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies


JoVE 3926

, , , , , ,

The Feinstein Institute for Medical Research, North Shore – LIJ Health System

Date Published: April 11, 2012  |  doi:10.3791/3926  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Herbal therapies, innate immune cells, cytokines, HMGB1, experimental animal model of sepsis, cecal ligation and puncture

 JoVE Clinical and Translational Medicine

Stereotactic Radiosurgery for Gynecologic Cancer


JoVE 3793

1, 1, 2

1Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine, 2Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine

Date Published: April 17, 2012  |  doi:10.3791/3793  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, radiosurgery, Cyberknife stereotactic radiosurgery, radiation, ovarian cancer, cervix cancer

 JoVE Clinical and Translational Medicine

The Polyvinyl Alcohol Sponge Model Implantation


JoVE 3885

1,2, 1,2, 1,2,3

1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, 2The Department of Veterans Affairs Medical Center, 3Internal Medicine, Vanderbilt University School of Medicine

Date Published: April 18, 2012  |  doi:10.3791/3885  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Polyvinyl alcohol (PVA) sponge, engraftment, stem cells, granulation tissue, vascularization, tumorgenesis, drug delivery, wound model, physiology

 JoVE Clinical and Translational Medicine

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System


JoVE 3956

1, 2, 1, 2, 1

1Department of Radiology, Brain Imaging Center, Academic Medical Center Amsterdam, 2Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College London

Date Published: April 25, 2012  |  doi:10.3791/3956  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Pharmacological MRI, Neuroscience, rat, 5-HT, BOLD, translational imaging, brain, fMRI

 JoVE Clinical and Translational Medicine

Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)


JoVE 3918

1, 1, 1, 1, 1,2

1Department of Neurosurgery, The University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Date Published: April 29, 2012  |  doi:10.3791/3918  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Label-Retaining Cells, Slow-Dividing Cells, Cancer Stem Cells, Glioblastoma, CFSE

 JoVE Bioengineering

Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis


JoVE 3654

1, 1, 1, 1, 1, 1, 2

1Department of Chemical Engineering and Chemical Technology, South Kensington campus, Imperial College London, 2Department of Hematology, Northwick Park & St. Mark's campus, Imperial College London

Date Published: April 10, 2012  |  doi:10.3791/3654  |  Section: JoVE Bioengineering

Keywords: Bioengineering, three-dimensional culture, hematopoiesis, leukemia, cord blood

 JoVE Bioengineering

Microfluidic Mixers for Studying Protein Folding


JoVE 3976

1, 1, 2, 3, 1

1Department of Physics and Astronomy, Michigan State University, 2Department of Mechanical Engineering, Hong Kong University of Science and Technology, 3Center for Biophotonics, University of California, Davis

Date Published: April 10, 2012  |  doi:10.3791/3976  |  Section: JoVE Bioengineering

Keywords: Bioengineering, microfluidic mixing, laminar flow, protein folding, fluorescence, FRET

 JoVE Bioengineering

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery


JoVE 3876

1,2, 1, 1, 1, 1, 1, 1

1Department of Nanomedicine, The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology

Date Published: April 17, 2012  |  doi:10.3791/3876  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Nanoporous silica chip, Low molecular weight proteomics, Peptidomics, MALDI-TOF mass spectrometry, early diagnostics, proteomics

 JoVE Bioengineering

Silk Film Culture System for in vitro Analysis and Biomaterial Design


JoVE 3646

1, 1, 1, 2, 1

1Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College, 2Department of Biomedical Engineering, Tufts University

Date Published: April 24, 2012  |  doi:10.3791/3646  |  Section: JoVE Bioengineering

Keywords: Bioengineering, silk, fibroin, film, biomaterial, surface patterning, in vitro, epithelium, cell culture

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