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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Essentials of
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Table of Contents

Table of Contents: Issue 62, April 2012

Jump to:  JoVE Editorial (1) JoVE Biology (17) JoVE Neuroscience (10) JoVE Immunology and Infection (10) JoVE Clinical and Translational Medicine (10) JoVE Bioengineering (4) JoVE Errata (6)
 JoVE Biology

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

1Basic Medical Sciences, University of Arizona College of Medicine - Phoenix


JoVE 3868

Date Published: April 3, 2012  |  doi:10.3791/3868  |  Section: JoVE Biology

 JoVE Biology

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

1Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, 2Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, 3Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud


JoVE 3513

Date Published: April 6, 2012  |  doi:10.3791/3513  |  Section: JoVE Biology

 JoVE Biology

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

1Biosphere Oriented Biology Research Unit, RIKEN Advanced Science Institute, 2Graduate School of Nanobioscience, Yokohama City University, 3Advanced NMR Metabomics Research Team, RIKEN Plant Science Center, 4Graduate School of Bioagricultural Science, Nagoya University


JoVE 3163

Date Published: April 7, 2012  |  doi:10.3791/3163  |  Section: JoVE Biology

 JoVE Biology

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

1Herman B Wells Center for Pediatric Research, Indiana University School of Medicine


JoVE 3872

Date Published: April 13, 2012  |  doi:10.3791/3872  |  Section: JoVE Biology

 JoVE Biology

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

1Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, 2Regional Agency for Environmental Protection in Emilia-Romagna


JoVE 3790

Date Published: April 14, 2012  |  doi:10.3791/3790  |  Section: JoVE Biology

 JoVE Biology

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

1Department of Microbiology, Miami University


JoVE 3992

Date Published: April 20, 2012  |  doi:10.3791/3992  |  Section: JoVE Biology

 JoVE Biology

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine


JoVE 3850

Date Published: April 24, 2012  |  doi:10.3791/3850  |  Section: JoVE Biology

 JoVE Biology

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

1Howard Hughes Medical Institute, Department of Neurobiology, Duke University, 2Department of Biological Sciences, Hokkaido University


JoVE 3764

Date Published: April 27, 2012  |  doi:10.3791/3764  |  Section: JoVE Biology

 JoVE Biology

The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity

1Department of Physiology and Pharmacology, Center for Cardiovascular and Respiratory Sciences, West Virginia University


JoVE 3674

Date Published: April 28, 2012  |  doi:10.3791/3674  |  Section: JoVE Biology

 JoVE Biology

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore


JoVE 3770

Date Published: April 30, 2012  |  doi:10.3791/3770  |  Section: JoVE Biology

 JoVE Neuroscience

Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique

1Monell Chemical Senses Center, 2Department of Physiology, Development & Neuroscience, Physiological Laboratory, University of Cambridge


JoVE 3862

Date Published: April 5, 2012  |  doi:10.3791/3862  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

1Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary


JoVE 4042

Date Published: April 5, 2012  |  doi:10.3791/4042  |  Section: JoVE Neuroscience

 JoVE Neuroscience

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

1Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK, 2Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK


JoVE 3920

Date Published: April 12, 2012  |  doi:10.3791/3920  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

1Department of Biological Sciences, Florida Atlantic University, 2Department of Chemistry & Biochemistry, Florida Atlantic University


JoVE 3597

Date Published: April 15, 2012  |  doi:10.3791/3597  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

1Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health


JoVE 4008

Date Published: April 19, 2012  |  doi:10.3791/4008  |  Section: JoVE Neuroscience

 JoVE Neuroscience

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

1Department of Neurosurgery, The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran


JoVE 3712

Date Published: April 22, 2012  |  doi:10.3791/3712  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Nerve Excitability Assessment in Chemotherapy-induced Neurotoxicity

1Prince of Wales Clinical School, University of New South Wales, 2Neuroscience Research Australia, University of New South Wales, 3School of Medical Sciences, University of New South Wales


JoVE 3439

Date Published: April 26, 2012  |  doi:10.3791/3439  |  Section: JoVE Neuroscience

 JoVE Neuroscience

Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster

1Department of Neurobiology, University of Massachusetts Medical School


JoVE 3625

Date Published: April 26, 2012  |  doi:10.3791/3625  |  Section: JoVE Neuroscience

 JoVE Immunology and Infection

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University


JoVE 3819

Date Published: April 2, 2012  |  doi:10.3791/3819  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine


JoVE 3823

Date Published: April 2, 2012  |  doi:10.3791/3823  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

1System Biosciences


JoVE 3171

Date Published: April 8, 2012  |  doi:10.3791/3171  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

1Department of Medicine, Section of Cardiology, St. John-Providence Health System, 2Department of Immunology and Microbiology, Wayne State University School of Medicine


JoVE 3778

Date Published: April 9, 2012  |  doi:10.3791/3778  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

1Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky


JoVE 3738

Date Published: April 16, 2012  |  doi:10.3791/3738  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

1Department of Internal Medicine, Yale University School of Medicine


JoVE 3741

Date Published: April 16, 2012  |  doi:10.3791/3741  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

1Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, 2School of Agrarian Sciences, University of Antioquia


JoVE 3533

Date Published: April 21, 2012  |  doi:10.3791/3533  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ)


JoVE 3518

Date Published: April 23, 2012  |  doi:10.3791/3518  |  Section: JoVE Immunology and Infection

 JoVE Immunology and Infection

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

1Plant Pathology and Microbiology, Texas A&M University


JoVE 3727

Date Published: April 23, 2012  |  doi:10.3791/3727  |  Section: JoVE Immunology and Infection

 JoVE Clinical and Translational Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

1Center for Brain and Spinal Cord Repair, School of Allied Medical Professions, The Ohio State University, 2Spinal Cord Research Center, Drexel University College of Medicine


JoVE 3247

Date Published: April 4, 2012  |  doi:10.3791/3247  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

1Carestream Molecular Imaging, 2Department of Chemistry and Biochemistry, University of Notre Dame, 3Freimann Life Science Center, University of Notre Dame, 4Research and Development, Oncovision, GEM-Imaging S.A.


JoVE 3680

Date Published: April 4, 2012  |  doi:10.3791/3680  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects

1Department of Orthopaedic Surgery, University of California, San Francisco


JoVE 3552

Date Published: April 11, 2012  |  doi:10.3791/3552  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies

1The Feinstein Institute for Medical Research, North Shore – LIJ Health System


JoVE 3926

Date Published: April 11, 2012  |  doi:10.3791/3926  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Stereotactic Radiosurgery for Gynecologic Cancer

1Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine, 2Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine


JoVE 3793

Date Published: April 17, 2012  |  doi:10.3791/3793  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury

1Department of Biology, Unit of Zoology, University of Fribourg, Fribourg, Switzerland


JoVE 3666

Date Published: April 18, 2012  |  doi:10.3791/3666  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Polyvinyl Alcohol Sponge Model Implantation

1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, 2The Department of Veterans Affairs Medical Center, 3Internal Medicine, Vanderbilt University School of Medicine


JoVE 3885

Date Published: April 18, 2012  |  doi:10.3791/3885  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer

1Biomedical Sciences and Pharmacy, The University of Newcastle


JoVE 3689

Date Published: April 25, 2012  |  doi:10.3791/3689  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System

1Department of Radiology, Brain Imaging Center, Academic Medical Center Amsterdam, 2Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College London


JoVE 3956

Date Published: April 25, 2012  |  doi:10.3791/3956  |  Section: JoVE Clinical and Translational Medicine

 JoVE Clinical and Translational Medicine

Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)

1Department of Neurosurgery, The University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran


JoVE 3918

Date Published: April 29, 2012  |  doi:10.3791/3918  |  Section: JoVE Clinical and Translational Medicine

 JoVE Bioengineering

Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis

1Department of Chemical Engineering and Chemical Technology, South Kensington campus, Imperial College London, 2Department of Hematology, Northwick Park & St. Mark's campus, Imperial College London


JoVE 3654

Date Published: April 10, 2012  |  doi:10.3791/3654  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Microfluidic Mixers for Studying Protein Folding

1Department of Physics and Astronomy, Michigan State University, 2Department of Mechanical Engineering, Hong Kong University of Science and Technology, 3Center for Biophotonics, University of California, Davis


JoVE 3976

Date Published: April 10, 2012  |  doi:10.3791/3976  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

1Department of Nanomedicine, The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology


JoVE 3876

Date Published: April 17, 2012  |  doi:10.3791/3876  |  Section: JoVE Bioengineering

 JoVE Bioengineering

Silk Film Culture System for in vitro Analysis and Biomaterial Design

1Margaret M. Dyson Vision Research Institute, Weill Cornell Medical College, 2Department of Biomedical Engineering, Tufts University


JoVE 3646

Date Published: April 24, 2012  |  doi:10.3791/3646  |  Section: JoVE Bioengineering

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