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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Table of Contents

Table of Contents: Issue 63, May 2012

Jump to:  JoVE Editorial (1) JoVE General (20) JoVE Neuroscience (10) JoVE Immunology and Infection (8) JoVE Clinical and Translational Medicine (9) JoVE Bioengineering (7)
 JoVE General

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets


JoVE 3650

,

Department of Molecular Microbiology, University of Texas Southwestern Medical Center

Date Published: May 4, 2012  |  doi:10.3791/3650  |  Section: JoVE General

Keywords: Cellular Biology, Correlative light and electron microscopy, microinjection, subcellular localization, fluorescent microscopy, microscopy, CLEM

 JoVE General

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples


JoVE 3791

1, 1, 2, 1, 3, 4, 5

1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute, 5Institute for Hepatitis and Virus Research, Serome Biosciences Inc.

Date Published: May 4, 2012  |  doi:10.3791/3791  |  Section: JoVE General

Keywords: Molecular Biology, Glycoproteins, glycan-binding protein, specific protein glycosylation, multiplexed high-throughput glycan blocked antibody microarray

 JoVE General

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)


JoVE 4002

1,2, 1,3, 1,2,3

1Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, 2Department of Microbiology and Immunology, McGill University, 3Department of Medicine, Division of Experimental Medicine, McGill University

Date Published: May 5, 2012  |  doi:10.3791/4002  |  Section: JoVE General

Keywords: Genetics, Viral genomic RNA, Fluorescence in situ Hybridization, FISH, imaging, genomics

 JoVE General

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks


JoVE 3903

1,2, 1,2,3, 1,2

1Molecular and Microbiology Department and Center for the Study of Genomics in Liver Diseases, George Mason University, 2Translational Research Institute, Inova Health System, 3Research Center for Medical Genetics RAMS

Date Published: May 11, 2012  |  doi:10.3791/3903  |  Section: JoVE General

Keywords: Basic Protocols, Biological samples, Device, Liquid nitrogen, Dewar, Sample Retrieval

 JoVE General

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy


JoVE 3585

1, 2, 1,3

1Department of Biochemistry and Molecular Biology, LSU School of Medicine, 2Department of Oral Biology, LSU School of Dentistry, 3Stanley S. Scott Cancer Center, LSU School of Medicine

Date Published: May 13, 2012  |  doi:10.3791/3585  |  Section: JoVE General

Keywords: Cellular Biology, migration, real time, time lapse, video microscopy

 JoVE General

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil


JoVE 3767

1,2, 2, 2,3

1DOE-Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, 2Department of Soil Science, University of Wisconsin, Madison, 3Department of Soil and Water Science, University of Florida

Date Published: May 19, 2012  |  doi:10.3791/3767  |  Section: JoVE General

Keywords: Molecular Biology, Glucosamine, muramic acid, microbial residue, aldononitrile acetate derivatization, isotope incorporation, ion structure, electron ionization, GC, MS

 JoVE General

Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis


JoVE 3954

1, 1, 2, 3, 1

1Department of Biomedical Engineering, Tulane University, 2Department of Biomedical Engineering, University of Virginia, 3Center for Stem Cell Research and Regenerative Medicine, Tulane University

Date Published: May 20, 2012  |  doi:10.3791/3954  |  Section: JoVE General

Keywords: Cellular Biology, mesentery, rat, angiogenesis, microcirculation, microvascular, remodeling

 JoVE General

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies


JoVE 3998

Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles

Date Published: May 22, 2012  |  doi:10.3791/3998  |  Section: JoVE General

Keywords: Basic Protocols, PCR, optimization, primer design, melting temperature, Tm, troubleshooting, additives, enhancers, template DNA quantification, thermal cycler, molecular biology, genetics

 JoVE General

Blood Collection for Biochemical Analysis in Adult Zebrafish


JoVE 3865

1,2, 1,2, 1, 1, 1, 1,2

1Hospital de Clínicas de Porto Alegre, Centro de Pesquisa Experimental Laboratório de Hepatologia e Gastroenterologia Experimental, 2Universidade Federal do Rio Grande do Sul, UFRGS. Porto Alegre, RS, Brasil

Date Published: May 26, 2012  |  doi:10.3791/3865  |  Section: JoVE General

Keywords: Biochemistry, Developmental Biology, Zebrafish, Zebrafish blood, Hematologic, Biochemical analysis

 JoVE General

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)


JoVE 3599

1,2,3, 4,5,6, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3

1Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, 3Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, 4École Centrale Marseille, Technopôle de Château-Gombert, 5Institut Fresnel, Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University

Date Published: May 27, 2012  |  doi:10.3791/3599  |  Section: JoVE General

Keywords: Physics, Single-particle tracking, single-molecule fluorescence microscopy, image analysis, tracking algorithm, high-resolution diffusion map, plasma membrane lateral organization

 JoVE General

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example


JoVE 3937

, ,

Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel

Date Published: May 29, 2012  |  doi:10.3791/3937  |  Section: JoVE General

Keywords: Molecular Biology, Virology, Poliovirus, VHH, nanobody, magnetic beads, affinity capture, liquid phase based assay, protein interaction

 JoVE General

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas


JoVE 3620

, , , ,

Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University

Date Published: May 31, 2012  |  doi:10.3791/3620  |  Section: JoVE General

Keywords: Genetics, Immunology, Molecular Biology, tissue microarray, immunohistochemistry, slide scanning, the Human Protein Atlas, protein profiles

 JoVE Neuroscience

A Fully Automated and Highly Versatile System for Testing Multi-cognitive Functions and Recording Neuronal Activities in Rodents


JoVE 3685

,

The Neurosciences Institute, San Diego, CA

Date Published: May 3, 2012  |  doi:10.3791/3685  |  Section: JoVE Neuroscience

Keywords: Neuroscience, auditory behavioral task, acoustic chamber, cognition test, multi-channel recording, electrophysiology, attention, motivation, decision, patience, rat, two-alternative choice pitch discrimination task, behavior

 JoVE Neuroscience

MPI CyberMotion Simulator: Implementation of a Novel Motion Simulator to Investigate Multisensory Path Integration in Three Dimensions


JoVE 3436

1, 1, 2, 1, 1,3

1Department of Human Perception, Cognition and Action, Max Planck Institute for Biological Cybernetics, 2Laboratoire de Physiologie de la Perception et de l'Action, Collège de France - CNRS, 3Department of Brain and Cognitive Engineering, Korea University

Date Published: May 10, 2012  |  doi:10.3791/3436  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Motion simulator, multisensory integration, path integration, space perception, vestibular, vision, robotics, cybernetics

 JoVE Neuroscience

Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats


JoVE 3785

1, 2, 2, 1

1Department of Pharmacology and Toxicology, Wright State University, 2Human Movement Laboratory, Universidade São Judas Tadeu

Date Published: May 17, 2012  |  doi:10.3791/3785  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Diabetes, painful diabetic neuropathy, nociception, thermal nociceptive threshold, nocifensive behavior

 JoVE Neuroscience

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex


JoVE 3621

1,2,3, 1,2, 1,2, 1,2,3

1Department of Molecular, Cellular Biology and Biochemistry, Brown University, 2Institute for Brain Science, Brown University, 3Department of Psychiatry and Human Behavior, Warren Alpert School of Medicine, Brown University

Date Published: May 18, 2012  |  doi:10.3791/3621  |  Section: JoVE Neuroscience

Keywords: Neuroscience, electroporation, organotypic slices, RNAi, neurogenesis, neuronal migration, neuronal morphogenesis, brain

 JoVE Neuroscience

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae


JoVE 3837

*1, *1,2, 1,3,4

1Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, 2Scholars Academy/MARC Scholar, University of Houston-Downtown, 3Genes and Development Graduate Program, University of Texas Graduate School of Biomedical Sciences, 4Neuroscience Graduate Program, University of Texas Graduate School of Biomedical Sciences

Date Published: May 18, 2012  |  doi:10.3791/3837  |  Section: JoVE Neuroscience

Keywords: Neuroscience, Drosophila sensory neurons, thermal nociception, nociceptive sensitization, tissue damage, fly behavioral response, dendritic arborization neurons, allodynia, hyperalgesia, behavioral assay

 JoVE Immunology and Infection

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response


JoVE 3347

*1,2, *1,2, 1, 1,2

1Biology Department, The City College of New York, CUNY, 2The Graduate Center, The City University of New York

Date Published: May 7, 2012  |  doi:10.3791/3347  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Parasitoid wasps, innate immunity, encapsulation, hematopoiesis, insect, fat body, Toll-NF-kappaB, molecular biology

 JoVE Immunology and Infection

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus


JoVE 3627

1, 1,2, 3, 1,2,4,5

1Center for Cell and Gene Therapy, Baylor College of Medicine, 2Pathology and Immunology, Baylor College of Medicine, 3Department of Stem Cell Transplantation and Cellular Therapy, University of Texas M.D. Anderson Cancer Center, 4Medicine, Baylor College of Medicine, 5Department of Pediatrics, Baylor College of Medicine

Date Published: May 7, 2012  |  doi:10.3791/3627  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Cytotoxic T Lymphocytes (CTL), virus, stem cell transplantation, cord blood, naïve T cells, medicine

 JoVE Immunology and Infection

Murine Model of Allergen Induced Asthma


JoVE 3771

, ,

Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University and Atlanta VA Medical Center

Date Published: May 14, 2012  |  doi:10.3791/3771  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Allergy, airway hyperresponsiveness, pulmonary function, eosinophil, ovalbumin, methacholine, airway resistance, plethysmography, flexiVent, bronchoalveolar lavage, physiology

 JoVE Immunology and Infection

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes


JoVE 3986

, , ,

Department of Microbiology and Immunology, Pennsylvania State University College of Medicine

Date Published: May 14, 2012  |  doi:10.3791/3986  |  Section: JoVE Immunology and Infection

Keywords: Stem Cell Biology, Immunology, T cells, induced pluripotent stem cells, differentiation, Notch signaling, T cell receptor, adoptive cell transfer

 JoVE Immunology and Infection

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine


JoVE 4040

1, 1, 2, 1, 1,2

1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

Date Published: May 21, 2012  |  doi:10.3791/4040  |  Section: JoVE Immunology and Infection

Keywords: Immunology, intestine, immunology, APCs, dendritic cells, macrophages, cell culture

 JoVE Immunology and Infection

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction


JoVE 3916

,

Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

Date Published: May 28, 2012  |  doi:10.3791/3916  |  Section: JoVE Immunology and Infection

Keywords: Immunology, Microbiology, Gram-negative, LPS, extraction, polysaccharide staining, Western immunoblot

 JoVE Clinical and Translational Medicine

Quantification of Atherosclerotic Plaque Activity and Vascular Inflammation using [18-F] Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography (FDG-PET/CT)


JoVE 3777

1, 2, 3, 2, 2

1Division of Cardiovascular Medicine, University of Pennsylvania, Perelman School of Medicine, 2Department of Radiology, University of Pennsylvania, Perelman School of Medicine, 3Department of Dermatology, University of Pennsylvania, Perelman School of Medicine

Date Published: May 2, 2012  |  doi:10.3791/3777  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, FDG-PET/CT, atherosclerosis, vascular inflammation, quantitative radiology, imaging

 JoVE Clinical and Translational Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis


JoVE 3663

1,2, 1,2, 3, 3, 4, 4, 5, 5, 6, 1,2, 7, 1,2,7

1University Heart Center Hamburg, TSI-Lab, Germany, 2Cardiovascular Research Center, University of Hamburg, 3Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, 4Department of Medicine, Stanford University School of Medicine, 5Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, 6Translumina GmbH, Hechingen, 7Department of Cardiothoracic Surgery, Stanford University School of Medicine

Date Published: May 9, 2012  |  doi:10.3791/3663  |  Section: JoVE Clinical and Translational Medicine

Keywords: Biomedical Engineering, physiology, stent, Human Internal Mammary Artery (IMA) Transplantation, restenosis

 JoVE Clinical and Translational Medicine

A Murine Model of Muscle Training by Neuromuscular Electrical Stimulation


JoVE 3914

1,2,3, 2, 1,2, 1,2, 2

1Department of Physical Medicine and Rehabilitation, University of Pittsburgh, 2Department of Physical Therapy, University of Pittsburgh, 3McGowan Institute for Regenerative Medicine, University of Pittsburgh

Date Published: May 9, 2012  |  doi:10.3791/3914  |  Section: JoVE Clinical and Translational Medicine

Keywords: Neuroscience, Neuromuscular electrical stimulation, skeletal muscle, pre-clinical, animal, medicine, physiology

 JoVE Clinical and Translational Medicine

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)


JoVE 3874

1, 2, 3, 1, 1,3,4

1Department of Laboratory Medicine & Pathobiology, University of Toronto, 2Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences, Sunnybrook Research Institute

Date Published: May 16, 2012  |  doi:10.3791/3874  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, cancer, primer assay, Prostate, microRNA, tumor, qPCR

 JoVE Clinical and Translational Medicine

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva


JoVE 3999

1, 1, 1, 2, 3, 4, 4, 5, 5, 6

1Department of Medicine, Weill Cornell Medical College, 2Department of Oral Biology, University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology, University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital, Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry

Date Published: May 16, 2012  |  doi:10.3791/3999  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, CD4 count, saliva, antitrypsin, hematopoiesis, T cells, HIV/AIDS, clinical

 JoVE Clinical and Translational Medicine

Collection Protocol for Human Pancreas


JoVE 4039

, , , , , ,

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

Date Published: May 23, 2012  |  doi:10.3791/4039  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, pancreas, organ donor, endocrine cells, insulin, beta-cells, islet, type 1 diabetes, type 2 diabetes

 JoVE Clinical and Translational Medicine

Staining Protocols for Human Pancreatic Islets


JoVE 4068

, , , ,

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

Date Published: May 23, 2012  |  doi:10.3791/4068  |  Section: JoVE Clinical and Translational Medicine

Keywords: Medicine, Physiology, type 1 diabetes, histology, H&E, immunohistochemistry, insulin, beta-cells, glucagon, alpha-cells, pancreatic polypeptide, islet, pancreas, spleen, organ donor

 JoVE Clinical and Translational Medicine

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen


JoVE 3586

1, 2, 2, 1

1Department of Chemistry and Biochemistry, University of Windsor, 2Chemistry Department and Centre for Biotechnology, Brock University

Date Published: May 30, 2012  |  doi:10.3791/3586  |  Section: JoVE Clinical and Translational Medicine

Keywords: Cancer Biology, Medicine, Biochemistry, Breast adenocarcinoma, neuroblastoma, tamoxifen, combination therapy, apoptosis, autophagy

 JoVE Bioengineering

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy


JoVE 3982

1, 1, 1,2, 1

1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München

Date Published: May 1, 2012  |  doi:10.3791/3982  |  Section: JoVE Bioengineering

Keywords: Bioengineering, TIRF, imaging, yeast, bacteria, microscopy, cell cortex

 JoVE Bioengineering

Use of Human Perivascular Stem Cells for Bone Regeneration


JoVE 2952

*1, *2, 2, 1, 2, 1, 1, 1, 3, 1, 3, 1, 2,4, 2

1Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, 2UCLA and Orthopaedic Hospital, Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA, 3Department of Bioengineering, UCLA, 4Center for Cardiovascular Science, University of Edinburgh

Date Published: May 25, 2012  |  doi:10.3791/2952  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Stem Cell Biology, Pericyte, Stem Cell, Bone Defect, Tissue Engineering, Osteogenesis, femoral defect, calvarial defect

 JoVE Bioengineering

Engineering a Bilayered Hydrogel to Control ASC Differentiation


JoVE 3953

1, 1, 2, 1

1Department of Extremity Trauma Research and Regenerative Medicine, United States Army Institute of Surgical Research, 2Department of Biomedical Engineering, The University of Texas at Austin

Date Published: May 25, 2012  |  doi:10.3791/3953  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Biomedical Engineering, Tissue Engineering, chitosan, microspheres, collagen, hydrogel, PEG fibrin, cell delivery, adipose-derived stem cells, ASC, CSM

 JoVE Bioengineering

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy


JoVE 3977

1,2, 2, 2,3, 2,4, 1,2

1Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, 2Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, 3Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, 4Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany

Date Published: May 29, 2012  |  doi:10.3791/3977  |  Section: JoVE Bioengineering

Keywords: Bioengineering, Raman spectroscopy, label-free analysis, living cells, extracellular matrix, tissue engineering

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